Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8845 and 8846 are separated by 575 nucleotides8846 and 8847 are separated by 644 nucleotides8847 and 8848 are separated by 527 nucleotides8848 and 8849 are separated by 249 nucleotides mRNA_477: 8845 - K10256 FAD2 omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase), at 1,108,768 to 1,110,470 8845 mRNA_478: 8846 - HMMPfam-Tetratricopeptide repeat-PF07719,HMMPfam-TPR repeat-PF13414,ProSiteProfiles-TPR repeat region circular profile.-PS50293,SMART-Tetratricopeptide repeats-SM00028,SUPERFAMILY--SSF48452, at 1,111,046 to 1,113,487 8846 mRNA_479: 8847 - K17302 COPB2, SEC27 coatomer subunit beta', at 1,114,132 to 1,117,214 8847 mRNA_480: 8848 - ProSiteProfiles-Myb-type HTH DNA-binding domain profile.-PS51294,SUPERFAMILY--SSF46689, at 1,117,742 to 1,121,401 8848 mRNA_481: 8849 - K11756 RSC1_2 chromatin structure-remodeling complex subunit RSC1/2, at 1,121,651 to 1,124,733 8849
Group Condition mRNA_477 mRNA_478 mRNA_479 mRNA_480 mRNA_481
8845 8846 8847 8848 8849
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -0.4 -0.7
growth on ypd YPD N.D. -0.2 N.D. +0.1 -1.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 N.D. +0.4 -1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. -0.3 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. -0.4 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. -0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. +0.5 -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. +0.0 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. -0.2 -0.1
auxotrophy Dropout Complete N.D. +0.0 N.D. +0.5 -0.6
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. +0.4 -0.3
auxotrophy YNB Glucose N.D. +0.0 N.D. +0.6 -0.4
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 N.D. +0.5 -0.2
growth on oleic acid YNB Glucose N.D. +0.4 N.D. +0.3 +0.3
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