Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8853 and 8854 are separated by 190 nucleotides8854 and 8855 are separated by 283 nucleotides8855 and 8856 are separated by 216 nucleotides8856 and 8857 are separated by 1764 nucleotides mRNA_485: 8853 - K13278 ASPG 60kDa lysophospholipase, at 1,154,487 to 1,156,857 8853 mRNA_486: 8854 - HMMPfam-Domain of unknown function (DUF202)-PF02656, at 1,157,048 to 1,157,778 8854 mRNA_487: 8855 - HMMPfam-Thioredoxin-PF00085,HMMPfam-PITH domain-PF06201,PRINTS-Thioredoxin family signature-PR00421,ProSitePatterns-Thioredoxin family active site.-PS00194,ProSiteProfiles-Thioredoxin domain profile.-PS51352,ProSiteProfiles-PITH domain profile.-PS51532,SUPERFAMILY--SSF49785,SUPERFAMILY--SSF52833, at 1,158,062 to 1,159,223 8855 mRNA_488: 8856 - Hypothetical Protein, at 1,159,440 to 1,160,357 8856 mRNA_489: 8857 - K07179 RIOK2 RIO kinase 2, at 1,162,122 to 1,163,780 8857
Group Condition mRNA_485 mRNA_486 mRNA_487 mRNA_488 mRNA_489
8853 8854 8855 8856 8857
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -1.3 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -1.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.9 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -1.0 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.8 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -1.0 N.D. N.D. N.D.
growth on ypd YPD +0.1 -0.4 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.2 -0.6 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.4 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.3 -0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.4 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.3 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.2 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.5 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.6 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.1 +0.7 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.7 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 +1.3 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.2 +1.2 N.D. N.D. N.D.
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