Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8878 and 8879 are separated by 176 nucleotides8879 and 8880 are separated by 107 nucleotides8880 and 8881 are separated by 2062 nucleotides8881 and 8882 are separated by 938 nucleotides mRNA_510: 8878 - K01922 PPCS, coaB phosphopantothenate-cysteine ligase, at 1,214,489 to 1,216,016 8878 mRNA_511: 8879 - KOG1426 FOG RCC1 domain, at 1,216,193 to 1,218,102 8879 mRNA_512: 8880 - HMMPfam-Glutathione S-transferase, C-terminal domain-PF13410,HMMPfam-Glutathione S-transferase, N-terminal domain-PF13417,ProSiteProfiles-Soluble glutathione S-transferase N-terminal domain profile.-PS50404,ProSiteProfiles-Soluble glutathione S-transferase C-terminal domain profile.-PS50405,SUPERFAMILY--SSF47616,SUPERFAMILY--SSF52833, at 1,218,210 to 1,219,403 8880 mRNA_513: 8881 - K09468 MSN2_4 zinc finger protein MSN2/4, at 1,221,466 to 1,223,534 8881 mRNA_514: 8882 - KOG0417 Ubiquitin-protein ligase, at 1,224,473 to 1,225,235 8882
Group Condition mRNA_510 mRNA_511 mRNA_512 mRNA_513 mRNA_514
8878 8879 8880 8881 8882
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 +0.2 -0.2 -3.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.1 -0.1 -1.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 +0.3 +0.1 -1.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.1 +0.0 -1.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.0 +0.2 -1.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.0 +0.2 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 -0.2 +0.1 -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.5 -0.1 -0.5 -0.6
growth on ypd YPD N.D. +0.2 -0.0 +0.2 -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 -0.2 +0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.4 -0.0 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.1 +0.1 -0.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.0 +0.4 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.2 +0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 +0.2 +0.1 -0.5
auxotrophy Dropout Complete N.D. +0.3 +0.4 +0.5 -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.3 +0.5 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.1 +0.0 +0.7
auxotrophy YNB Glucose N.D. +0.3 +0.2 +0.4 -0.3
growth on oleic acid YNB Glucose N.D. +0.4 -0.3 +0.5 -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.1 +0.7 +0.6
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