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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8878 and 8879 are separated by 176 nucleotides
8879 and 8880 are separated by 107 nucleotides
8880 and 8881 are separated by 2062 nucleotides
8881 and 8882 are separated by 938 nucleotides
mRNA_510: 8878 - K01922 PPCS, coaB phosphopantothenate-cysteine ligase, at 1,214,489 to 1,216,016
8878
mRNA_511: 8879 - KOG1426 FOG RCC1 domain, at 1,216,193 to 1,218,102
8879
mRNA_512: 8880 - HMMPfam-Glutathione S-transferase, C-terminal domain-PF13410,HMMPfam-Glutathione S-transferase, N-terminal domain-PF13417,ProSiteProfiles-Soluble glutathione S-transferase N-terminal domain profile.-PS50404,ProSiteProfiles-Soluble glutathione S-transferase C-terminal domain profile.-PS50405,SUPERFAMILY--SSF47616,SUPERFAMILY--SSF52833, at 1,218,210 to 1,219,403
8880
mRNA_513: 8881 - K09468 MSN2_4 zinc finger protein MSN2/4, at 1,221,466 to 1,223,534
8881
mRNA_514: 8882 - KOG0417 Ubiquitin-protein ligase, at 1,224,473 to 1,225,235
8882
Group
Condition
mRNA
_510
mRNA
_511
mRNA
_512
mRNA
_513
mRNA
_514
8878
8879
8880
8881
8882
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
-0.5
+0.2
-0.2
-3.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.2
-0.1
-0.1
-1.3
auxotrophy
YNB Glucose 75mM Methionine
N.D.
+0.3
+0.3
+0.1
-1.9
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
+0.1
-0.1
+0.0
-1.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
-0.2
+0.0
+0.2
-1.0
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.2
+0.0
+0.2
-0.9
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
+0.0
-0.2
+0.1
-0.7
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
+0.5
-0.1
-0.5
-0.6
growth on ypd
YPD
N.D.
+0.2
-0.0
+0.2
-1.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
-0.4
-0.2
+0.3
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.2
-0.4
-0.0
+0.1
growth on ricinoleic acid
YNB Glucose
N.D.
+0.2
-0.1
+0.1
-0.6
auxotrophy
YNB Glucose 75mM Arginine
N.D.
+0.1
+0.0
+0.4
-0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
-0.2
-0.2
+0.4
+0.1
ypd buoyancy
40 hrs Median Buoyancy
N.D.
+0.3
+0.2
+0.1
-0.5
auxotrophy
Dropout Complete
N.D.
+0.3
+0.4
+0.5
-1.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+0.0
-0.3
+0.5
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-0.2
-0.1
+0.0
+0.7
auxotrophy
YNB Glucose
N.D.
+0.3
+0.2
+0.4
-0.3
growth on oleic acid
YNB Glucose
N.D.
+0.4
-0.3
+0.5
-0.0
growth on oleic acid
YNB Oleic Acid
N.D.
+0.2
+0.1
+0.7
+0.6
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