Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8880 and 8881 are separated by 2062 nucleotides8881 and 8882 are separated by 938 nucleotides8882 and 8883 are separated by 279 nucleotides8883 and 8884 are separated by 216 nucleotides mRNA_512: 8880 - HMMPfam-Glutathione S-transferase, C-terminal domain-PF13410,HMMPfam-Glutathione S-transferase, N-terminal domain-PF13417,ProSiteProfiles-Soluble glutathione S-transferase N-terminal domain profile.-PS50404,ProSiteProfiles-Soluble glutathione S-transferase C-terminal domain profile.-PS50405,SUPERFAMILY--SSF47616,SUPERFAMILY--SSF52833, at 1,218,210 to 1,219,403 8880 mRNA_513: 8881 - K09468 MSN2_4 zinc finger protein MSN2/4, at 1,221,466 to 1,223,534 8881 mRNA_514: 8882 - KOG0417 Ubiquitin-protein ligase, at 1,224,473 to 1,225,235 8882 mRNA_515: 8883 - Hypothetical Protein, at 1,225,515 to 1,227,526 8883 mRNA_516: 8884 - K04482 RAD51 DNA repair protein RAD51, at 1,227,743 to 1,229,231 8884
Group Condition mRNA_512 mRNA_513 mRNA_514 mRNA_515 mRNA_516
8880 8881 8882 8883 8884
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.2 -3.4 +0.1 -0.8
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 -0.9 -0.4 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 -1.3 -0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.5 -0.6 -0.7 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.1 -1.9 -0.3 +0.3
growth on ypd YPD -0.0 +0.2 -1.1 -0.7 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.0 +0.1 -0.9 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 -0.7 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.0 -1.1 +0.0 +0.1
auxotrophy Dropout Complete +0.4 +0.5 -1.0 -0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 -1.0 +0.0 +0.0
growth on ricinoleic acid YNB Glucose -0.1 +0.1 -0.6 -0.3 +0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.0 +0.7 -0.5 -0.5
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.4 -0.6 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 -0.5 -0.1 -0.1
growth on oleic acid YNB Glucose -0.3 +0.5 -0.0 -0.4 +0.4
auxotrophy YNB Glucose +0.2 +0.4 -0.3 +0.3 -0.0
growth on oleic acid YNB Oleic Acid +0.1 +0.7 +0.6 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.3 -0.3 +0.4 +1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.5 -0.1 +0.6 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.4 +0.1 +0.2 +0.9
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