Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8898 and 8899 are separated by 133 nucleotides8899 and 8900 are separated by 370 nucleotides8900 and 8901 are separated by 273 nucleotides8901 and 8902 are separated by 435 nucleotides mRNA_530: 8898 - Hypothetical Protein, at 1,264,038 to 1,264,727 8898 mRNA_531: 8899 - KOG1812 Predicted E3 ubiquitin ligase, at 1,264,861 to 1,266,270 8899 mRNA_532: 8900 - K05355 hexPS, COQ1 hexaprenyl-diphosphate synthase, at 1,266,641 to 1,268,317 8900 mRNA_533: 8901 - K17759 AIBP, nnrE NAD(P)H-hydrate epimerase, at 1,268,591 to 1,269,786 8901 mRNA_534: 8902 - K08054 CANX calnexin, at 1,270,222 to 1,272,470 8902
Group Condition mRNA_530 mRNA_531 mRNA_532 mRNA_533 mRNA_534
8898 8899 8900 8901 8902
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.0 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.2 -0.1
auxotrophy Dropout Complete N.D. N.D. N.D. +0.3 -0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.2 -0.0
growth on ypd YPD N.D. N.D. N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.0 +0.5
auxotrophy YNB Glucose N.D. N.D. N.D. +0.4 +0.2
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