Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8901 and 8902 are separated by 435 nucleotides8902 and 8903 are separated by 230 nucleotides8903 and 8904 are separated by 205 nucleotides8904 and 8905 are separated by 401 nucleotides mRNA_533: 8901 - K17759 AIBP, nnrE NAD(P)H-hydrate epimerase, at 1,268,591 to 1,269,786 8901 mRNA_534: 8902 - K08054 CANX calnexin, at 1,270,222 to 1,272,470 8902 mRNA_535: 8903 - HMMPfam-Ribosomal protein S21-PF01165,TIGRFAM-S21p ribosomal protein S21-TIGR00030, at 1,272,701 to 1,273,396 8903 mRNA_536: 8904 - K09548 PFDN1 prefoldin subunit 1, at 1,273,602 to 1,274,230 8904 mRNA_537: 8905 - KOG0725 Reductases with broad range of substrate specificities, at 1,274,632 to 1,275,903 8905
Group Condition mRNA_533 mRNA_534 mRNA_535 mRNA_536 mRNA_537
8901 8902 8903 8904 8905
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.1 N.D. -1.8 -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.4 N.D. -1.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.3 N.D. -1.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -1.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.3 N.D. -0.8 +0.1
auxotrophy Dropout Complete +0.3 -0.1 N.D. -1.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 N.D. -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 N.D. -0.5 +0.3
growth on oleic acid YNB Glucose +0.2 -0.0 N.D. -0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.2 -0.0 N.D. +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.1 N.D. -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.4 -0.2
growth on ypd YPD +0.1 +0.1 N.D. -0.5 +0.4
auxotrophy YNB Glucose +0.4 +0.2 N.D. -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 N.D. +0.3 +0.1
growth on oleic acid YNB Oleic Acid +0.1 +0.4 N.D. -0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.2 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. +0.3 +0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.5 N.D. +0.7 +0.2
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