Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8909 and 8910 are separated by 223 nucleotides8910 and 8911 are separated by 275 nucleotides8911 and 8912 are separated by 362 nucleotides8912 and 8913 are separated by 574 nucleotides mRNA_541: 8909 - HMMPfam-NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8)-PF05821, at 1,286,793 to 1,287,579 8909 mRNA_542: 8910 - KOG0118 FOG RRM domain, at 1,287,803 to 1,289,304 8910 mRNA_543: 8911 - K14838 NOP15 nucleolar protein 15, at 1,289,580 to 1,290,883 8911 mRNA_544: 8912 - K00902 E2.7.1.108 dolichol kinase, at 1,291,246 to 1,294,493 8912 mRNA_545: 8913 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 1,295,068 to 1,298,428 8913
Group Condition mRNA_541 mRNA_542 mRNA_543 mRNA_544 mRNA_545
8909 8910 8911 8912 8913
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. N.D. -0.6
auxotrophy Dropout Complete N.D. N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. N.D. -0.2
growth on ypd YPD N.D. N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. N.D. -0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. N.D. +0.0
auxotrophy YNB Glucose N.D. N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. N.D. +0.3
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