Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8912 and 8913 are separated by 574 nucleotides8913 and 8914 are separated by 229 nucleotides8914 and 8915 are separated by 251 nucleotides8915 and 8916 are separated by 575 nucleotides mRNA_544: 8912 - K00902 E2.7.1.108 dolichol kinase, at 1,291,246 to 1,294,493 8912 mRNA_545: 8913 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 1,295,068 to 1,298,428 8913 mRNA_546: 8914 - K00799 GST, gst glutathione S-transferase, at 1,298,658 to 1,299,726 8914 mRNA_547: 8915 - KOG2385 Uncharacterized conserved protein, at 1,299,978 to 1,303,835 8915 mRNA_548: 8916 - K15084 SLC25A16, GDA, LEU5 solute carrier family 25 (mitochondrial carrier protein), member 16, at 1,304,411 to 1,305,873 8916
Group Condition mRNA_544 mRNA_545 mRNA_546 mRNA_547 mRNA_548
8912 8913 8914 8915 8916
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.4 -1.8 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 +0.4 -1.9 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.3 -1.1 N.D.
auxotrophy Dropout Complete N.D. -0.3 -0.3 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -0.2 -0.6 N.D.
auxotrophy YNB Glucose N.D. +0.1 -0.6 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 +0.1 -0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.6 -0.5 +0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.2 -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.5 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.2 -0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.2 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 +0.6 -0.6 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 -0.5 +0.7 N.D.
growth on oleic acid YNB Glucose N.D. -0.0 -0.4 +0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.4 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.6 +1.2 N.D.
growth on ypd YPD N.D. -0.2 +0.8 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 +0.6 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 +0.3 +0.6 N.D.
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