Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8914 and 8915 are separated by 251 nucleotides8915 and 8916 are separated by 575 nucleotides8916 and 8917 are separated by 732 nucleotides8917 and 8918 are separated by 189 nucleotides mRNA_546: 8914 - K00799 GST, gst glutathione S-transferase, at 1,298,658 to 1,299,726 8914 mRNA_547: 8915 - KOG2385 Uncharacterized conserved protein, at 1,299,978 to 1,303,835 8915 mRNA_548: 8916 - K15084 SLC25A16, GDA, LEU5 solute carrier family 25 (mitochondrial carrier protein), member 16, at 1,304,411 to 1,305,873 8916 mRNA_549: 8917 - K12618 XRN1, SEP1, KEM1 5'-3' exoribonuclease 1, at 1,306,606 to 1,311,810 8917 mRNA_550: 8918 - KOG4391 Predicted alpha/beta hydrolase BEM46, at 1,312,000 to 1,313,154 8918
Group Condition mRNA_546 mRNA_547 mRNA_548 mRNA_549 mRNA_550
8914 8915 8916 8917 8918
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -1.9 N.D. -0.6 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.4 -1.8 N.D. +0.0 +0.2
auxotrophy YNB Glucose -0.6 -0.2 N.D. -0.7 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.5 +0.2 N.D. -1.0 -0.2
growth on oleic acid YNB Oleic Acid -0.2 -0.2 N.D. -0.0 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.3 -1.1 N.D. +0.3 +0.1
auxotrophy Dropout Complete -0.3 -0.4 N.D. +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.6 +1.2 N.D. -1.1 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.6 N.D. +0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.5 N.D. -0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 N.D. -1.1 +0.1
growth on oleic acid YNB Glucose -0.4 +0.7 N.D. -0.5 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.6 N.D. -0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. +0.3 -0.0
growth on ricinoleic acid YNB Glucose -0.5 +0.7 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.6 N.D. +0.7 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.6 N.D. +1.1 +0.5
growth on ypd YPD +0.8 +0.3 N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.3 N.D. +0.8 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.1 N.D. +1.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.6 N.D. +1.0 +0.2
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