Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8919 and 8920 are separated by 237 nucleotides8920 and 8921 are separated by 200 nucleotides8921 and 8922 are separated by 1009 nucleotides8922 and 8923 are separated by 445 nucleotides mRNA_551: 8919 - KOG1515 Arylacetamide deacetylase, at 1,313,966 to 1,316,695 8919 mRNA_552: 8920 - KOG1761 Signal recognition particle, subunit Srp14, at 1,316,933 to 1,317,920 8920 mRNA_553: 8921 - KOG0907 Thioredoxin, at 1,318,121 to 1,318,450 8921 mRNA_554: 8922 - K13337 PEX19 peroxin-19, at 1,319,460 to 1,321,018 8922 mRNA_555: 8923 - K03115 CSNK2B casein kinase II subunit beta, at 1,321,464 to 1,322,533 8923
Group Condition mRNA_551 mRNA_552 mRNA_553 mRNA_554 mRNA_555
8919 8920 8921 8922 8923
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -1.8 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.3 -0.5 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -1.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.9 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.5 -0.2 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.5 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.6 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.5 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.1 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.2 -0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.2 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.5 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.3 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.0 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.2 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.6 -0.3 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.2 +0.1 N.D. N.D. N.D.
growth on ypd YPD -0.1 +0.5 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.1 N.D. N.D. N.D.
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