Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8923 and 8924 are separated by 288 nucleotides8924 and 8925 are separated by 728 nucleotides8925 and 8926 are separated by 834 nucleotides8926 and 8927 are separated by 645 nucleotides mRNA_555: 8923 - K03115 CSNK2B casein kinase II subunit beta, at 1,321,464 to 1,322,533 8923 mRNA_556: 8924 - HMMPfam-Acetyltransferase (GNAT) domain-PF13302,SUPERFAMILY--SSF55729, at 1,322,822 to 1,323,921 8924 mRNA_557: 8925 - HMMPfam-Ras association (RalGDS/AF-6) domain-PF00788,ProSiteProfiles-Ras-associating (RA) domain profile.-PS50200,SMART-Ras association (RalGDS/AF-6) domain-SM00314,SUPERFAMILY--SSF54236, at 1,324,650 to 1,329,010 8925 mRNA_558: 8926 - HMMPfam-SH3 domain-PF00018,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044, at 1,329,845 to 1,332,752 8926 mRNA_559: 8927 - KOG4672 Uncharacterized conserved low complexity protein, at 1,333,398 to 1,335,053 8927
Group Condition mRNA_555 mRNA_556 mRNA_557 mRNA_558 mRNA_559
8923 8924 8925 8926 8927
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 +0.0 N.D. -2.8
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.8 +0.3 N.D. -1.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 +0.1 N.D. -0.7
auxotrophy YNB Glucose N.D. -0.2 +0.5 N.D. -1.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 -0.2 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.0 N.D. -0.7
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 +0.3 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.4 +0.2 N.D. +0.0
growth on ypd YPD N.D. -0.3 +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 +0.3 N.D. -0.2
auxotrophy Dropout Complete N.D. -0.1 +0.2 N.D. +0.0
growth on oleic acid YNB Glucose N.D. -0.4 +0.2 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.5 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +1.0 N.D. -0.5
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.5 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 +0.6 N.D. +0.5
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.4 N.D. +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.9 +0.5 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 +1.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +1.2 +1.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 +0.8 N.D. +1.0
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