Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8925 and 8926 are separated by 834 nucleotides8926 and 8927 are separated by 645 nucleotides8927 and 8928 are separated by 1430 nucleotides8928 and 8929 are separated by 355 nucleotides mRNA_557: 8925 - HMMPfam-Ras association (RalGDS/AF-6) domain-PF00788,ProSiteProfiles-Ras-associating (RA) domain profile.-PS50200,SMART-Ras association (RalGDS/AF-6) domain-SM00314,SUPERFAMILY--SSF54236, at 1,324,650 to 1,329,010 8925 mRNA_558: 8926 - HMMPfam-SH3 domain-PF00018,ProSiteProfiles-Src homology 3 (SH3) domain profile.-PS50002,SMART-Src homology 3 domains-SM00326,SUPERFAMILY--SSF50044, at 1,329,845 to 1,332,752 8926 mRNA_559: 8927 - KOG4672 Uncharacterized conserved low complexity protein, at 1,333,398 to 1,335,053 8927 mRNA_560: 8928 - Hypothetical Protein, at 1,336,484 to 1,340,949 8928 mRNA_561: 8929 - Hypothetical Protein, at 1,341,305 to 1,346,099 8929
Group Condition mRNA_557 mRNA_558 mRNA_559 mRNA_560 mRNA_561
8925 8926 8927 8928 8929
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. -2.8 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -1.4 -0.0 -0.4
auxotrophy YNB Glucose +0.5 N.D. -1.2 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2 +0.0 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.7 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.7 +0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.4 -0.1 +0.0
auxotrophy Dropout Complete +0.2 N.D. +0.0 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.2 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.0 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. +0.2 -0.1 -0.3
growth on oleic acid YNB Glucose +0.2 N.D. +0.4 -0.2 -0.0
growth on ypd YPD +0.2 N.D. +0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.0 N.D. -0.3 +0.0 -0.2
growth on ricinoleic acid YNB Glucose +0.5 N.D. +0.2 -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. -0.0 +0.3 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +1.0 N.D. -0.5 -0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. +0.5 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 N.D. -0.1 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 N.D. +1.0 -0.1 -0.3
growth on oleic acid YNB Oleic Acid +0.4 N.D. +0.9 +0.1 +0.0
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