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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8942 and 8943 are separated by 337 nucleotides
8943 and 8944 are separated by 286 nucleotides
8944 and 8945 are separated by 176 nucleotides
8945 and 8946 are separated by 328 nucleotides
mRNA_574: 8942 - K02999 RPA1, POLR1A DNA-directed RNA polymerase I subunit RPA1, at 1,372,563 to 1,378,346
8942
mRNA_575: 8943 - K00940 ndk, NME nucleoside-diphosphate kinase, at 1,378,684 to 1,379,466
8943
mRNA_576: 8944 - K20182 VPS33 vacuolar protein sorting-associated protein 33, at 1,379,753 to 1,382,507
8944
mRNA_577: 8945 - Hypothetical Protein, at 1,382,684 to 1,384,433
8945
mRNA_578: 8946 - K05687 PARK7 protein DJ-1, at 1,384,762 to 1,386,533
8946
Group
Condition
mRNA
_574
mRNA
_575
mRNA
_576
mRNA
_577
mRNA
_578
8942
8943
8944
8945
8946
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
-2.9
+0.2
-2.6
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
-2.2
-0.5
+0.3
growth on ypd
YPD
N.D.
N.D.
-1.0
-0.1
-0.6
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
-0.6
+0.3
-0.4
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
-1.0
+0.4
-0.1
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
-0.5
+0.4
-0.4
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
-1.1
+0.5
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
-0.4
+0.2
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
-0.7
+0.2
+0.2
auxotrophy
Dropout Complete
N.D.
N.D.
-0.4
+0.6
-0.2
growth on oleic acid
YNB Glucose
N.D.
N.D.
+0.1
+0.1
-0.2
auxotrophy
YNB Glucose
N.D.
N.D.
-0.2
+0.5
-0.1
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
+0.2
+0.4
-0.3
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
-0.5
+0.6
+0.3
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
-0.3
+0.2
+0.6
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
-0.7
+0.1
+1.2
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
+0.3
+0.4
+0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
+0.3
+0.4
+0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
-0.5
+0.2
+1.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
+0.1
+0.2
+1.5
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.2
-0.1
+2.2
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