Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8956 and 8957 are separated by 4105 nucleotides8957 and 8958 are separated by 274 nucleotides8958 and 8959 are separated by 114 nucleotides8959 and 8960 are separated by 126 nucleotides mRNA_588: 8956 - K12609 CAF120 CCR4-NOT transcriptional complex subunit CAF120, at 1,406,393 to 1,408,614 8956 mRNA_589: 8957 - KOG1763 Uncharacterized conserved protein, contains CCCH-type Zn-finger, at 1,412,720 to 1,414,364 8957 mRNA_590: 8958 - K05543 DUS2 tRNA-dihydrouridine synthase 2, at 1,414,639 to 1,416,336 8958 mRNA_591: 8959 - K01915 glnA, GLUL glutamine synthetase, at 1,416,451 to 1,418,413 8959 mRNA_592: 8960 - K10747 LIG1 DNA ligase 1, at 1,418,540 to 1,422,145 8960
Group Condition mRNA_588 mRNA_589 mRNA_590 mRNA_591 mRNA_592
8956 8957 8958 8959 8960
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.7 N.D. -0.5 -0.7
auxotrophy Dropout Complete -0.4 -0.7 N.D. -0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.4 N.D. -0.6 -0.3
growth on ypd YPD -0.3 -0.6 N.D. +0.1 -0.2
growth on oleic acid YNB Glucose -0.2 -0.7 N.D. -0.1 +0.1
auxotrophy YNB Glucose -0.2 -0.2 N.D. -0.5 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.3 N.D. -0.1 -0.3
growth on oleic acid YNB Oleic Acid -0.2 +0.1 N.D. -0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.1 N.D. -0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.5 N.D. -0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.1 N.D. -0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.4 N.D. -0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.0 N.D. -0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.4 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.4 N.D. -0.2 -0.0
growth on ricinoleic acid YNB Glucose -0.3 +0.3 N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.5 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.8 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.4 N.D. +0.4 +0.0
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