Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8957 and 8958 are separated by 274 nucleotides8958 and 8959 are separated by 114 nucleotides8959 and 8960 are separated by 126 nucleotides8960 and 8961 are separated by 334 nucleotides mRNA_589: 8957 - KOG1763 Uncharacterized conserved protein, contains CCCH-type Zn-finger, at 1,412,720 to 1,414,364 8957 mRNA_590: 8958 - K05543 DUS2 tRNA-dihydrouridine synthase 2, at 1,414,639 to 1,416,336 8958 mRNA_591: 8959 - K01915 glnA, GLUL glutamine synthetase, at 1,416,451 to 1,418,413 8959 mRNA_592: 8960 - K10747 LIG1 DNA ligase 1, at 1,418,540 to 1,422,145 8960 mRNA_593: 8961 - K18469 TBC1D5 TBC1 domain family member 5, at 1,422,480 to 1,425,082 8961
Group Condition mRNA_589 mRNA_590 mRNA_591 mRNA_592 mRNA_593
8957 8958 8959 8960 8961
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. -0.5 -0.7 -1.1
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.6 -0.3 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. -0.2 +0.0 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.2 +0.0 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. -0.1 -0.3 -0.2
auxotrophy Dropout Complete -0.7 N.D. -0.2 +0.0 -0.0
growth on ypd YPD -0.6 N.D. +0.1 -0.2 +0.0
auxotrophy YNB Glucose -0.2 N.D. -0.5 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.1 -0.3 +0.2
growth on oleic acid YNB Glucose -0.7 N.D. -0.1 +0.1 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.5 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. -0.4 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.2 -0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.0 +0.0 -0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.4 -0.1 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. -0.5 -0.1 +0.7
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.3 +0.2 +0.6
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 N.D. -0.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +0.4 +0.0 -0.0
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