Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8968 and 8969 are separated by 428 nucleotides8969 and 8970 are separated by 277 nucleotides8970 and 8971 are separated by 554 nucleotides8971 and 8972 are separated by 387 nucleotides mRNA_600: 8968 - K17785 IMMT, FCJ1, MNOS2 mitofilin, at 1,443,273 to 1,445,582 8968 mRNA_601: 8969 - K01784 galE, GALE UDP-glucose 4-epimerase, at 1,446,011 to 1,447,734 8969 mRNA_602: 8970 - K14642 YND1 golgi apyrase, at 1,448,012 to 1,450,852 8970 mRNA_603: 8971 - Hypothetical Protein, at 1,451,407 to 1,452,135 8971 mRNA_604: 8972 - K17871 ndh1 NADH-ubiquinone reductase (non-electrogenic), at 1,452,523 to 1,454,931 8972
Group Condition mRNA_600 mRNA_601 mRNA_602 mRNA_603 mRNA_604
8968 8969 8970 8971 8972
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. +0.3 N.D. -2.3
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. -0.2 N.D. -1.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. -0.4 N.D. -1.3
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. +0.3 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. -0.3 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.2 N.D. -0.7
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.5 N.D. -0.8
growth on ypd YPD -0.1 N.D. -0.5 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. +0.5 N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.7 N.D. +0.6 N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 N.D. -0.8
auxotrophy YNB Glucose -0.7 N.D. +0.6 N.D. -0.4
growth on oleic acid YNB Glucose -0.5 N.D. +0.5 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.5 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.7 N.D. +0.4 N.D. +0.3
auxotrophy Dropout Complete -0.3 N.D. +0.5 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.6 N.D. +1.9 N.D. +0.5
growth on oleic acid YNB Oleic Acid -0.5 N.D. +0.9 N.D. +1.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.4 N.D. +1.4 N.D. +2.1
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