Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8972 and 8973 are separated by 348 nucleotides8973 and 8974 are separated by 998 nucleotides8974 and 8975 are separated by 269 nucleotides8975 and 8976 are separated by 1301 nucleotides mRNA_604: 8972 - K17871 ndh1 NADH-ubiquinone reductase (non-electrogenic), at 1,452,523 to 1,454,931 8972 mRNA_605: 8973 - SUPERFAMILY--SSF52047, at 1,455,280 to 1,456,250 8973 mRNA_606: 8974 - K06867 K06867 uncharacterized protein, at 1,457,249 to 1,458,122 8974 mRNA_607: 8975 - K00140 mmsA, iolA, ALDH6A1 malonate-semialdehyde dehydrogenase (acetylating) / methylmalonate-semialdehyde dehydrogenase, at 1,458,392 to 1,460,964 8975 mRNA_608: 8976 - K09490 HSPA5, BIP heat shock 70kDa protein 5, at 1,462,266 to 1,464,196 8976
Group Condition mRNA_604 mRNA_605 mRNA_606 mRNA_607 mRNA_608
8972 8973 8974 8975 8976
facs on lipid accumulation Sorted Low BODIPY Gate -2.3 -0.0 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 -0.4 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.3 -0.0 N.D. -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.3 N.D. -0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -1.1 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.8 -0.2 N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -0.4 -0.4 N.D. -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.4 +0.1 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 -0.0 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.5 -0.0 N.D. -0.3 N.D.
auxotrophy YNB Glucose -0.4 -0.0 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.1 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.2 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.0 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.2 N.D. -0.0 N.D.
auxotrophy Dropout Complete -0.1 +0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.2 N.D. +0.1 N.D.
growth on ypd YPD +0.0 +0.4 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.6 N.D. -0.4 N.D.
growth on oleic acid YNB Oleic Acid +1.2 -0.0 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +2.1 +0.3 N.D. -1.1 N.D.
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