Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8977 and 8978 are separated by 277 nucleotides8978 and 8979 are separated by 70 nucleotides8979 and 8980 are separated by 109 nucleotides8980 and 8981 are separated by 147 nucleotides mRNA_609: 8977 - K19001 HELLS, DDM1 ATP-dependent DNA helicase, at 1,464,752 to 1,468,761 8977 mRNA_610: 8978 - K06961 KRR1 ribosomal RNA assembly protein, at 1,469,039 to 1,470,636 8978 mRNA_611: 8979 - K00490 CYP4F cytochrome P450, family 4, subfamily F, at 1,470,707 to 1,472,980 8979 mRNA_612: 8980 - KOG3183 Predicted Zn-finger protein, at 1,473,090 to 1,474,198 8980 mRNA_613: 8981 - K10631 TOPORS E3 ubiquitin-protein ligase Topors, at 1,474,346 to 1,477,518 8981
Group Condition mRNA_609 mRNA_610 mRNA_611 mRNA_612 mRNA_613
8977 8978 8979 8980 8981
auxotrophy Dropout Complete +0.2 N.D. -1.3 -0.2 -1.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.4 -0.5 -1.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -1.1 -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. -0.4 +0.2 -0.7
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.2 -0.2 -0.8
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.3 -0.1 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.7 -0.5 -0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.3 -0.4 -0.5
auxotrophy YNB Glucose +0.2 N.D. -0.1 -0.1 -0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.5 +0.1 -0.5
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.0 +0.3 -0.6
growth on oleic acid YNB Glucose -0.0 N.D. +0.4 -0.1 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.3 -0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.3 -0.1 +0.0
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.2 +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.0 +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.2 -0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.7 -0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 N.D. +0.6 +0.5 -0.1
growth on ypd YPD +0.0 N.D. +0.6 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.1 +0.1 +0.1
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