Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8996 and 8998 are separated by 944 nucleotides8998 and 8999 are separated by 873 nucleotides8999 and 9000 are separated by 1045 nucleotides9000 and 9001 are separated by 272 nucleotides mRNA_628: 8996 - K17894 GATA2 GATA-binding protein 2, at 1,506,988 to 1,510,110 8996 mRNA_630: 8998 - K10088 OS9 protein OS-9, at 1,511,055 to 1,513,202 8998 mRNA_631: 8999 - Hypothetical Protein, at 1,514,076 to 1,515,222 8999 mRNA_632: 9000 - K17900 ATG15, AUT5 lipase ATG15, at 1,516,268 to 1,517,647 9000 mRNA_633: 9001 - K14575 AFG2, DRG1, SPATA5 AAA family ATPase, at 1,517,920 to 1,520,918 9001
Group Condition mRNA_628 mRNA_630 mRNA_631 mRNA_632 mRNA_633
8996 8998 8999 9000 9001
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.1 +0.4 -5.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.9 +0.1 -0.0 -3.9 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +2.5 -0.4 -0.2 -5.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 -0.2 -1.9 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 +0.1 -0.3 -1.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.0 +0.2 -0.3 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 +0.2 -0.0 -1.3 N.D.
growth on ricinoleic acid YNB Glucose -2.1 +0.5 +0.0 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.9 -0.1 -0.1 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.5 +0.2 -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 +0.6 +0.0 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.3 -0.1 -0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.3 -0.1 +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.9 -0.1 +0.6 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.9 +0.3 +0.5 +0.2 N.D.
auxotrophy YNB Glucose -0.9 +0.4 +0.6 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 +0.1 +0.1 N.D.
auxotrophy Dropout Complete -0.3 +0.1 +0.4 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.7 +0.3 +0.2 -0.5 N.D.
growth on oleic acid YNB Glucose -0.5 +0.4 +0.5 +0.3 N.D.
growth on ypd YPD +0.4 -0.1 +0.2 +0.4 N.D.
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