Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9000 and 9001 are separated by 272 nucleotides9001 and 9002 are separated by 94 nucleotides9002 and 9003 are separated by 3972 nucleotides9003 and 9004 are separated by 122 nucleotides mRNA_632: 9000 - K17900 ATG15, AUT5 lipase ATG15, at 1,516,268 to 1,517,647 9000 mRNA_633: 9001 - K14575 AFG2, DRG1, SPATA5 AAA family ATPase, at 1,517,920 to 1,520,918 9001 mRNA_634: 9002 - K18932 ZDHHC palmitoyltransferase, at 1,521,013 to 1,522,610 9002 mRNA_635: 9003 - KOG1470 Phosphatidylinositol transfer protein PDR16 and related proteins, at 1,526,583 to 1,528,432 9003 mRNA_636: 9004 - Hypothetical Protein, at 1,528,555 to 1,529,690 9004
Group Condition mRNA_632 mRNA_633 mRNA_634 mRNA_635 mRNA_636
9000 9001 9002 9003 9004
facs on lipid accumulation Sorted No BODIPY Gate -5.4 N.D. -0.1 +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -5.3 N.D. +0.4 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -3.9 N.D. +0.1 -0.1 -1.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.9 N.D. -0.2 -1.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -1.3 N.D. -0.3 -1.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 N.D. -0.2 -0.7 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.5 N.D. +0.1 -0.8 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 N.D. +0.2 -0.9 -0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.0 -0.9 -0.1
growth on ypd YPD +0.4 N.D. +0.3 -1.0 -0.5
growth on oleic acid YNB Glucose +0.3 N.D. -0.2 -0.6 -0.2
growth on oleic acid YNB Oleic Acid -0.5 N.D. +0.1 -0.4 +0.2
auxotrophy Dropout Complete +0.1 N.D. +0.2 -1.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. -0.1 -0.4 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.1 -0.8 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.1 -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.1 -0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.0 -0.9 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. -0.1 +0.6 +0.1
auxotrophy YNB Glucose +0.1 N.D. +0.1 -1.0 +1.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.4 +0.5
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