Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9004 and 9005 are separated by 108 nucleotides9005 and 9006 are separated by 470 nucleotides9006 and 9007 are separated by 794 nucleotides9007 and 9008 are separated by 181 nucleotides mRNA_636: 9004 - Hypothetical Protein, at 1,528,555 to 1,529,690 9004 mRNA_637: 9005 - K02516 PRMT5, HSL7 type II protein arginine methyltransferase, at 1,529,799 to 1,532,897 9005 mRNA_638: 9006 - K02947 RP-S10e, RPS10 small subunit ribosomal protein S10e, at 1,533,368 to 1,534,142 9006 mRNA_639: 9007 - K18027 PTPN3, PTPH1 tyrosine-protein phosphatase non-receptor type 3, at 1,534,937 to 1,536,294 9007 mRNA_640: 9008 - HMMPfam-UV radiation resistance protein and autophagy-related subunit 14-PF10186, at 1,536,476 to 1,538,269 9008
Group Condition mRNA_636 mRNA_637 mRNA_638 mRNA_639 mRNA_640
9004 9005 9006 9007 9008
facs on lipid accumulation Sorted High BODIPY Gate -1.3 N.D. N.D. +0.2 -4.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. +0.3 -2.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.2 -1.4
growth on ypd YPD -0.5 N.D. N.D. +0.1 -0.9
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. -0.0 -0.7
auxotrophy Dropout Complete +0.3 N.D. N.D. -0.1 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. -0.2 +0.3
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. +0.3 -0.5
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. -0.2 +0.7
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. -0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. N.D. -0.0 +0.0
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. -0.0 +0.6
auxotrophy YNB Glucose +1.0 N.D. N.D. -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. N.D. -0.0 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. +0.2 +0.7
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.1 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +0.3 +1.0
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. N.D. +0.1 +0.8
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