Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9007 and 9008 are separated by 181 nucleotides9008 and 9009 are separated by 122 nucleotides9009 and 9010 are separated by 338 nucleotides9010 and 9011 are separated by 169 nucleotides mRNA_639: 9007 - K18027 PTPN3, PTPH1 tyrosine-protein phosphatase non-receptor type 3, at 1,534,937 to 1,536,294 9007 mRNA_640: 9008 - HMMPfam-UV radiation resistance protein and autophagy-related subunit 14-PF10186, at 1,536,476 to 1,538,269 9008 mRNA_641: 9009 - K08333 PIK3R4, VPS15 phosphoinositide-3-kinase, regulatory subunit 4, at 1,538,392 to 1,544,328 9009 mRNA_642: 9010 - HMMPfam-Basic region leucine zipper-PF07716,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 1,544,667 to 1,546,033 9010 mRNA_643: 9011 - K15117 SLC25A34_35, OAC1 solute carrier family 25, member 34/35, at 1,546,203 to 1,548,129 9011
Group Condition mRNA_639 mRNA_640 mRNA_641 mRNA_642 mRNA_643
9007 9008 9009 9010 9011
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -4.0 -0.0 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -2.0 -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -1.4 -0.3 N.D. -0.2
growth on ypd YPD +0.1 -0.9 +0.3 N.D. -0.1
auxotrophy Dropout Complete -0.1 -0.4 +0.1 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.3 -0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.7 +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.5 -0.5 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.0 +0.3 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.5 -0.1 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 +0.2 N.D. +0.3
auxotrophy YNB Glucose -0.1 -0.3 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.7 +0.3 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.7 -0.0 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +1.0 -0.7 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.5 +0.4 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.0 +0.3 +0.6 N.D. -0.0
growth on ricinoleic acid YNB Glucose -0.0 +0.6 +0.3 N.D. -0.1
growth on oleic acid YNB Glucose -0.0 +0.2 +0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.7 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.8 +0.1 N.D. +0.0
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