Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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Top 21 experiments (either direction), sorted by average fitness

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500 nt 9009 and 9010 are separated by 338 nucleotides9010 and 9011 are separated by 169 nucleotides9011 and 9012 are separated by 446 nucleotides9012 and 9013 are separated by 453 nucleotides mRNA_641: 9009 - K08333 PIK3R4, VPS15 phosphoinositide-3-kinase, regulatory subunit 4, at 1,538,392 to 1,544,328 9009 mRNA_642: 9010 - HMMPfam-Basic region leucine zipper-PF07716,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 1,544,667 to 1,546,033 9010 mRNA_643: 9011 - K15117 SLC25A34_35, OAC1 solute carrier family 25, member 34/35, at 1,546,203 to 1,548,129 9011 mRNA_644: 9012 - KOG3020 TatD-related DNase, at 1,548,576 to 1,549,928 9012 mRNA_645: 9013 - KOG3104 Mod5 protein sorting/negative effector of RNA Pol III synthesis, at 1,550,382 to 1,551,455 9013
Group Condition mRNA_641 mRNA_642 mRNA_643 mRNA_644 mRNA_645
9009 9010 9011 9012 9013
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.3 -0.2 -2.8
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.2 +0.1 -1.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.2 -0.1 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.3 -0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.4 +0.0 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. -0.0 +0.4 +0.2
auxotrophy Dropout Complete +0.1 N.D. +0.1 -0.1 -0.1
auxotrophy YNB Glucose +0.2 N.D. +0.3 -0.1 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.3 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.0 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.2 -0.4 +1.4
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.2 -0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.4 +0.0 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.2 -0.2 +1.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.5 -0.1 +0.9
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.1 -0.2 +0.9
growth on ypd YPD +0.3 N.D. -0.1 -0.1 +1.1
growth on oleic acid YNB Glucose +0.2 N.D. +0.4 -0.1 +0.6
growth on oleic acid YNB Oleic Acid +0.6 N.D. -0.0 -0.1 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. +0.4 -0.3 +1.9
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.3 -0.1 +1.7
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