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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9009 and 9010 are separated by 338 nucleotides
9010 and 9011 are separated by 169 nucleotides
9011 and 9012 are separated by 446 nucleotides
9012 and 9013 are separated by 453 nucleotides
mRNA_641: 9009 - K08333 PIK3R4, VPS15 phosphoinositide-3-kinase, regulatory subunit 4, at 1,538,392 to 1,544,328
9009
mRNA_642: 9010 - HMMPfam-Basic region leucine zipper-PF07716,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 1,544,667 to 1,546,033
9010
mRNA_643: 9011 - K15117 SLC25A34_35, OAC1 solute carrier family 25, member 34/35, at 1,546,203 to 1,548,129
9011
mRNA_644: 9012 - KOG3020 TatD-related DNase, at 1,548,576 to 1,549,928
9012
mRNA_645: 9013 - KOG3104 Mod5 protein sorting/negative effector of RNA Pol III synthesis, at 1,550,382 to 1,551,455
9013
Group
Condition
mRNA
_641
mRNA
_642
mRNA
_643
mRNA
_644
mRNA
_645
9009
9010
9011
9012
9013
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
N.D.
-0.3
-0.2
-2.8
facs on lipid accumulation
Sorted No BODIPY Gate
-0.3
N.D.
-0.2
+0.1
-1.4
facs on lipid accumulation
Sorted High BODIPY Gate
-0.0
N.D.
-0.2
-0.1
-0.8
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
N.D.
-0.3
-0.3
+0.3
ypd buoyancy
40 hrs High Buoyancy
+0.3
N.D.
-0.4
+0.0
-0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.7
N.D.
-0.0
+0.4
+0.2
auxotrophy
Dropout Complete
+0.1
N.D.
+0.1
-0.1
-0.1
auxotrophy
YNB Glucose
+0.2
N.D.
+0.3
-0.1
-0.4
auxotrophy
YNB Glucose 75mM Arginine
+0.2
N.D.
+0.3
+0.1
-0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.1
N.D.
+0.0
-0.0
+0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.3
N.D.
-0.2
-0.4
+1.4
auxotrophy
YNB Glucose 75mM Methionine
+0.2
N.D.
+0.2
-0.0
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.4
N.D.
-0.4
+0.0
+0.6
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.0
N.D.
-0.2
-0.2
+1.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
-0.5
-0.1
+0.9
growth on ricinoleic acid
YNB Glucose
+0.3
N.D.
-0.1
-0.2
+0.9
growth on ypd
YPD
+0.3
N.D.
-0.1
-0.1
+1.1
growth on oleic acid
YNB Glucose
+0.2
N.D.
+0.4
-0.1
+0.6
growth on oleic acid
YNB Oleic Acid
+0.6
N.D.
-0.0
-0.1
+0.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.5
N.D.
+0.4
-0.3
+1.9
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
N.D.
+0.3
-0.1
+1.7
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