Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9019 and 9020 are separated by 1085 nucleotides9020 and 9021 are separated by 294 nucleotides9021 and 9022 are separated by 508 nucleotides9022 and 9023 are separated by 283 nucleotides mRNA_651: 9019 - Hypothetical Protein, at 1,568,205 to 1,569,102 9019 mRNA_652: 9020 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 1,570,188 to 1,571,223 9020 mRNA_653: 9021 - HMMPfam-RTA1 like protein-PF04479, at 1,571,518 to 1,572,791 9021 mRNA_654: 9022 - K14809 DDX55, SPB4 ATP-dependent RNA helicase DDX55/SPB4, at 1,573,300 to 1,575,930 9022 mRNA_655: 9023 - K11246 SHO1 SHO1 osmosensor, at 1,576,214 to 1,578,020 9023
Group Condition mRNA_651 mRNA_652 mRNA_653 mRNA_654 mRNA_655
9019 9020 9021 9022 9023
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.5 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.0 N.D. -0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 -0.6 N.D. -0.2
growth on oleic acid YNB Glucose N.D. -0.5 +0.3 N.D. -0.3
growth on ypd YPD N.D. -0.4 -0.1 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 +0.2 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 +0.2 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.0 N.D. +0.6
auxotrophy Dropout Complete N.D. -0.2 +0.6 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 +0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.3 N.D. +0.1
auxotrophy YNB Glucose N.D. +0.2 +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 +0.2 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.8 +0.7 N.D. -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.6 N.D. +0.1
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