Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9029 and 9030 are separated by 352 nucleotides9030 and 9031 are separated by 338 nucleotides9031 and 9032 are separated by 363 nucleotides9032 and 9033 are separated by 260 nucleotides mRNA_661: 9029 - Hypothetical Protein, at 1,593,045 to 1,593,501 9029 mRNA_662: 9030 - HMMPfam-EF-hand domain pair-PF13499,ProSitePatterns-EF-hand calcium-binding domain.-PS00018,ProSiteProfiles-EF-hand calcium-binding domain profile.-PS50222,SUPERFAMILY--SSF47473, at 1,593,854 to 1,595,354 9030 mRNA_663: 9031 - SUPERFAMILY--SSF103196, at 1,595,693 to 1,596,287 9031 mRNA_664: 9032 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13532,SUPERFAMILY--SSF51197, at 1,596,651 to 1,602,461 9032 mRNA_665: 9033 - K08853 AAK AP2-associated kinase, at 1,602,722 to 1,607,021 9033
Group Condition mRNA_661 mRNA_662 mRNA_663 mRNA_664 mRNA_665
9029 9030 9031 9032 9033
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.0 N.D. -0.3 -2.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 N.D. -0.2 -1.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.0 N.D. -0.2 -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 N.D. +0.1 -0.8
auxotrophy Dropout Complete +0.1 +0.4 N.D. -0.1 -1.0
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.5 N.D. +0.0 -1.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 N.D. +0.0 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 N.D. -0.1 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.5 N.D. -0.4 -0.8
auxotrophy YNB Glucose +0.1 +0.6 N.D. +0.1 -1.1
growth on ricinoleic acid YNB Glucose +0.1 +0.5 N.D. -0.0 -0.9
growth on oleic acid YNB Glucose +0.3 +0.2 N.D. +0.1 -0.8
growth on oleic acid YNB Oleic Acid +0.2 +0.3 N.D. -0.0 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.4 N.D. +0.3 -0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.3 N.D. -0.0 -0.1
growth on ypd YPD -0.0 +0.2 N.D. +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.5 N.D. +0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.6 N.D. -0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.6 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.4 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.2 N.D. -0.0 +0.1
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