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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9030 and 9031 are separated by 338 nucleotides
9031 and 9032 are separated by 363 nucleotides
9032 and 9033 are separated by 260 nucleotides
9033 and 9034 are separated by 60 nucleotides
mRNA_662: 9030 - HMMPfam-EF-hand domain pair-PF13499,ProSitePatterns-EF-hand calcium-binding domain.-PS00018,ProSiteProfiles-EF-hand calcium-binding domain profile.-PS50222,SUPERFAMILY--SSF47473, at 1,593,854 to 1,595,354
9030
mRNA_663: 9031 - SUPERFAMILY--SSF103196, at 1,595,693 to 1,596,287
9031
mRNA_664: 9032 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13532,SUPERFAMILY--SSF51197, at 1,596,651 to 1,602,461
9032
mRNA_665: 9033 - K08853 AAK AP2-associated kinase, at 1,602,722 to 1,607,021
9033
mRNA_666: 9034 - ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SUPERFAMILY--SSF57701, at 1,607,082 to 1,608,088
9034
Group
Condition
mRNA
_662
mRNA
_663
mRNA
_664
mRNA
_665
mRNA
_666
9030
9031
9032
9033
9034
auxotrophy
Dropout Complete
+0.4
N.D.
-0.1
-1.0
-1.1
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.0
N.D.
-0.3
-2.0
+0.5
facs on lipid accumulation
Sorted High BODIPY Gate
+0.5
N.D.
-0.4
-0.8
-0.8
auxotrophy
YNB Glucose 75mM Arginine
+0.2
N.D.
+0.0
-0.9
-0.7
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
N.D.
-0.2
-1.3
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.0
N.D.
-0.1
-0.4
-0.5
auxotrophy
YNB Glucose 75mM Methionine
+0.5
N.D.
+0.0
-1.2
-0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
N.D.
+0.1
-0.8
-0.1
auxotrophy
YNB Glucose
+0.6
N.D.
+0.1
-1.1
-0.5
growth on oleic acid
YNB Glucose
+0.2
N.D.
+0.1
-0.8
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.0
N.D.
-0.2
-1.0
+0.9
growth on ricinoleic acid
YNB Glucose
+0.5
N.D.
-0.0
-0.9
+0.3
growth on oleic acid
YNB Oleic Acid
+0.3
N.D.
-0.0
-0.5
+0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.3
N.D.
-0.0
-0.1
-0.1
growth on ypd
YPD
+0.2
N.D.
+0.2
-0.3
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.4
N.D.
+0.3
-0.8
+0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.5
N.D.
+0.1
-0.5
+0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.2
N.D.
-0.0
+0.1
+0.1
ypd buoyancy
40 hrs High Buoyancy
+0.6
N.D.
-0.1
-0.0
+0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.6
N.D.
+0.0
-0.1
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.4
N.D.
+0.0
+0.0
+0.2
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