Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9031 and 9032 are separated by 363 nucleotides9032 and 9033 are separated by 260 nucleotides9033 and 9034 are separated by 60 nucleotides9034 and 9035 are separated by 111 nucleotides mRNA_663: 9031 - SUPERFAMILY--SSF103196, at 1,595,693 to 1,596,287 9031 mRNA_664: 9032 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13532,SUPERFAMILY--SSF51197, at 1,596,651 to 1,602,461 9032 mRNA_665: 9033 - K08853 AAK AP2-associated kinase, at 1,602,722 to 1,607,021 9033 mRNA_666: 9034 - ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SUPERFAMILY--SSF57701, at 1,607,082 to 1,608,088 9034 mRNA_667: 9035 - Interpro Ribosome associated membrane protein RAMP4, at 1,608,200 to 1,608,918 9035
Group Condition mRNA_663 mRNA_664 mRNA_665 mRNA_666 mRNA_667
9031 9032 9033 9034 9035
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 -2.0 +0.5 -0.8
auxotrophy Dropout Complete N.D. -0.1 -1.0 -1.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 -0.8 -0.8 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -1.2 -0.4 -0.7
auxotrophy YNB Glucose N.D. +0.1 -1.1 -0.5 -0.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.9 -0.7 -0.3
growth on oleic acid YNB Glucose N.D. +0.1 -0.8 +0.0 -0.9
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -1.3 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.4 -0.5 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.5 +0.2 -0.4
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.9 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.8 -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.8 +0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.5 +0.3 -0.2
growth on ypd YPD N.D. +0.2 -0.3 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 -0.1 -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -1.0 +0.9 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.0 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.1 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 +0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.0 +0.2 +0.5
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