Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9036 and 9037 are separated by 667 nucleotides9037 and 9038 are separated by 873 nucleotides9038 and 9039 are separated by 326 nucleotides9039 and 9040 are separated by 63 nucleotides mRNA_668: 9036 - HMMPfam-F-box-like-PF12937,SUPERFAMILY--SSF52047, at 1,609,196 to 1,610,811 9036 mRNA_669: 9037 - K01689 ENO, eno enolase, at 1,611,479 to 1,614,227 9037 mRNA_670: 9038 - Hypothetical Protein, at 1,615,101 to 1,618,146 9038 mRNA_671: 9039 - K03846 ALG9 alpha-1,2-mannosyltransferase, at 1,618,473 to 1,620,853 9039 mRNA_672: 9040 - Hypothetical Protein, at 1,620,917 to 1,621,557 9040
Group Condition mRNA_668 mRNA_669 mRNA_670 mRNA_671 mRNA_672
9036 9037 9038 9039 9040
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. +0.0 -1.2 -0.6
auxotrophy Dropout Complete -0.1 N.D. -0.1 -0.4 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 -0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. +0.1 -0.5 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 +0.1 -0.4
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.1 +0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.4 -0.6 -0.0
growth on ypd YPD +0.2 N.D. -0.4 +0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.1 +0.2 -0.3
auxotrophy YNB Glucose +0.1 N.D. +0.1 -0.4 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.0 -0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.2 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.2 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.0 +0.1 +0.2
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.1 +0.3 +0.4
growth on oleic acid YNB Glucose +0.2 N.D. +0.1 +0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.4 +0.3 -0.0
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