Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9047 and 9048 are separated by 184 nucleotides9048 and 9049 are separated by 252 nucleotides9049 and 9050 are separated by 131 nucleotides9050 and 9051 are separated by 553 nucleotides mRNA_679: 9047 - Hypothetical Protein, at 1,629,434 to 1,632,180 9047 mRNA_680: 9048 - K03094 SKP1, CBF3D S-phase kinase-associated protein 1, at 1,632,365 to 1,633,590 9048 mRNA_681: 9049 - K00987 CEG1 mRNA guanylyltransferase, at 1,633,843 to 1,636,121 9049 mRNA_682: 9050 - K01487 E3.5.4.3, guaD guanine deaminase, at 1,636,253 to 1,638,256 9050 mRNA_683: 9051 - K01613 psd, PISD phosphatidylserine decarboxylase, at 1,638,810 to 1,640,262 9051
Group Condition mRNA_679 mRNA_680 mRNA_681 mRNA_682 mRNA_683
9047 9048 9049 9050 9051
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.2 -1.0
growth on ypd YPD N.D. N.D. N.D. -0.7 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.0 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 -0.3
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.3 -0.2
auxotrophy Dropout Complete N.D. N.D. N.D. -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.6 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.2 -0.0
auxotrophy YNB Glucose N.D. N.D. N.D. +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.5 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 +0.5
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.7 +0.2
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