Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9049 and 9050 are separated by 131 nucleotides9050 and 9051 are separated by 553 nucleotides9051 and 9052 are separated by 157 nucleotides9052 and 9053 are separated by 333 nucleotides mRNA_681: 9049 - K00987 CEG1 mRNA guanylyltransferase, at 1,633,843 to 1,636,121 9049 mRNA_682: 9050 - K01487 E3.5.4.3, guaD guanine deaminase, at 1,636,253 to 1,638,256 9050 mRNA_683: 9051 - K01613 psd, PISD phosphatidylserine decarboxylase, at 1,638,810 to 1,640,262 9051 mRNA_684: 9052 - HMMPfam-Nucleotide-diphospho-sugar transferase-PF03407, at 1,640,420 to 1,643,110 9052 mRNA_685: 9053 - K10577 UBE2I, UBC9 ubiquitin-conjugating enzyme E2 I, at 1,643,444 to 1,644,500 9053
Group Condition mRNA_681 mRNA_682 mRNA_683 mRNA_684 mRNA_685
9049 9050 9051 9052 9053
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -1.0 -0.1 N.D.
growth on ypd YPD N.D. -0.7 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.5 +0.0 N.D.
growth on oleic acid YNB Glucose N.D. -0.3 -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 -0.6 +0.1 N.D.
auxotrophy Dropout Complete N.D. -0.3 -0.1 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 -0.1 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 +0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.3 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -0.0 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 +0.4 -0.2 N.D.
auxotrophy YNB Glucose N.D. +0.0 -0.2 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.3 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.3 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.1 -0.1 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.3 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.3 +0.5 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.4 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 -0.0 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.2 +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.7 +0.2 -0.0 N.D.
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