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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9049 and 9050 are separated by 131 nucleotides
9050 and 9051 are separated by 553 nucleotides
9051 and 9052 are separated by 157 nucleotides
9052 and 9053 are separated by 333 nucleotides
mRNA_681: 9049 - K00987 CEG1 mRNA guanylyltransferase, at 1,633,843 to 1,636,121
9049
mRNA_682: 9050 - K01487 E3.5.4.3, guaD guanine deaminase, at 1,636,253 to 1,638,256
9050
mRNA_683: 9051 - K01613 psd, PISD phosphatidylserine decarboxylase, at 1,638,810 to 1,640,262
9051
mRNA_684: 9052 - HMMPfam-Nucleotide-diphospho-sugar transferase-PF03407, at 1,640,420 to 1,643,110
9052
mRNA_685: 9053 - K10577 UBE2I, UBC9 ubiquitin-conjugating enzyme E2 I, at 1,643,444 to 1,644,500
9053
Group
Condition
mRNA
_681
mRNA
_682
mRNA
_683
mRNA
_684
mRNA
_685
9049
9050
9051
9052
9053
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.2
-1.0
-0.1
N.D.
growth on ypd
YPD
N.D.
-0.7
-0.2
+0.1
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
-0.1
-0.5
+0.0
N.D.
growth on oleic acid
YNB Glucose
N.D.
-0.3
-0.2
-0.1
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
-0.0
-0.6
+0.1
N.D.
auxotrophy
Dropout Complete
N.D.
-0.3
-0.1
-0.1
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-0.4
-0.1
+0.1
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
-0.6
+0.3
-0.0
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
-0.1
-0.3
+0.1
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
-0.2
-0.0
+0.0
N.D.
ypd buoyancy
40 hrs Low Buoyancy
N.D.
-0.5
+0.4
-0.2
N.D.
auxotrophy
YNB Glucose
N.D.
+0.0
-0.2
-0.0
N.D.
auxotrophy
YNB Glucose 75mM Methionine
N.D.
+0.2
-0.3
+0.0
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.1
-0.3
+0.1
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.0
+0.1
-0.1
N.D.
growth on oleic acid
YNB Oleic Acid
N.D.
-0.1
+0.3
-0.2
N.D.
ypd buoyancy
40 hrs Median Buoyancy
N.D.
-0.3
+0.5
-0.1
N.D.
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.1
+0.4
-0.0
N.D.
auxotrophy
YNB Glucose 75mM Arginine
N.D.
+0.1
+0.2
-0.0
N.D.
growth on ricinoleic acid
YNB Glucose
N.D.
+0.1
+0.2
+0.0
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
+0.7
+0.2
-0.0
N.D.
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