Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9052 and 9053 are separated by 333 nucleotides9053 and 9054 are separated by 513 nucleotides9054 and 9055 are separated by 599 nucleotides9055 and 9056 are separated by 390 nucleotides mRNA_684: 9052 - HMMPfam-Nucleotide-diphospho-sugar transferase-PF03407, at 1,640,420 to 1,643,110 9052 mRNA_685: 9053 - K10577 UBE2I, UBC9 ubiquitin-conjugating enzyme E2 I, at 1,643,444 to 1,644,500 9053 mRNA_686: 9054 - K06867 K06867 uncharacterized protein, at 1,645,014 to 1,645,916 9054 mRNA_687: 9055 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,646,516 to 1,647,919 9055 mRNA_688: 9056 - Hypothetical Protein, at 1,648,310 to 1,648,900 9056
Group Condition mRNA_684 mRNA_685 mRNA_686 mRNA_687 mRNA_688
9052 9053 9054 9055 9056
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. -0.4 +0.1 -1.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.0 +0.1 -1.1
auxotrophy YNB Glucose -0.0 N.D. +0.1 -0.3 -0.6
growth on oleic acid YNB Glucose -0.1 N.D. -0.3 +0.0 -0.3
auxotrophy Dropout Complete -0.1 N.D. +0.1 -0.3 -0.4
growth on ricinoleic acid YNB Glucose +0.0 N.D. -0.2 +0.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.4 -0.5 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.1 +0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.0 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.1 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.2 +0.2 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.1 +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.2 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.1 +0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.1 -0.4 +0.5
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.1 +0.0 +0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. +0.6 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.2 +0.2 +0.7
growth on ypd YPD +0.1 N.D. -0.2 +0.8 +0.7
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