Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9064 and 9065 are separated by 221 nucleotides9065 and 9066 are separated by 682 nucleotides9066 and 9067 are separated by 234 nucleotides9067 and 9068 are separated by 220 nucleotides mRNA_696: 9064 - K03754 EIF2B2 translation initiation factor eIF-2B subunit beta, at 1,663,500 to 1,665,283 9064 mRNA_697: 9065 - KOG1389 3-oxoacyl CoA thiolase, at 1,665,505 to 1,667,348 9065 mRNA_698: 9066 - K12188 SNF8, EAP30 ESCRT-II complex subunit VPS22, at 1,668,031 to 1,668,867 9066 mRNA_699: 9067 - K20289 COG2 conserved oligomeric Golgi complex subunit 2, at 1,669,102 to 1,672,155 9067 mRNA_700: 9068 - K12398 AP3M AP-3 complex subunit mu, at 1,672,376 to 1,674,226 9068
Group Condition mRNA_696 mRNA_697 mRNA_698 mRNA_699 mRNA_700
9064 9065 9066 9067 9068
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -5.9 -0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 -5.7 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 -5.5 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -3.4 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 -3.4 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -2.4 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -1.1 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 -1.6 +0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.3 -3.4 +2.4 N.D.
growth on ypd YPD N.D. -0.2 -0.7 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -2.2 +1.8 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.1 -0.2 N.D.
growth on oleic acid YNB Glucose N.D. -0.2 -1.2 +1.4 N.D.
auxotrophy YNB Glucose N.D. +0.3 -2.0 +2.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -1.7 +1.9 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -3.5 +3.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.5 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.5 +0.7 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.4 +2.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.2 -2.7 +3.9 N.D.
auxotrophy Dropout Complete N.D. +0.4 -0.7 +2.0 N.D.
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