Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9065 and 9066 are separated by 682 nucleotides9066 and 9067 are separated by 234 nucleotides9067 and 9068 are separated by 220 nucleotides9068 and 9069 are separated by 100 nucleotides mRNA_697: 9065 - KOG1389 3-oxoacyl CoA thiolase, at 1,665,505 to 1,667,348 9065 mRNA_698: 9066 - K12188 SNF8, EAP30 ESCRT-II complex subunit VPS22, at 1,668,031 to 1,668,867 9066 mRNA_699: 9067 - K20289 COG2 conserved oligomeric Golgi complex subunit 2, at 1,669,102 to 1,672,155 9067 mRNA_700: 9068 - K12398 AP3M AP-3 complex subunit mu, at 1,672,376 to 1,674,226 9068 mRNA_701: 9069 - K08776 NPEPPS puromycin-sensitive aminopeptidase, at 1,674,327 to 1,678,341 9069
Group Condition mRNA_697 mRNA_698 mRNA_699 mRNA_700 mRNA_701
9065 9066 9067 9068 9069
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -5.7 -0.2 N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -5.9 -0.4 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -5.5 +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -3.4 +0.6 N.D. -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -3.4 +0.4 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -2.4 +0.4 N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -1.6 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -1.1 -0.4 N.D. -0.1
growth on ypd YPD -0.2 -0.7 +0.6 N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.3 -3.4 +2.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -2.2 +1.8 N.D. +0.1
growth on oleic acid YNB Glucose -0.2 -1.2 +1.4 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -0.2 N.D. +0.1
auxotrophy YNB Glucose +0.3 -2.0 +2.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -3.5 +3.8 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 -1.7 +1.9 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.5 +0.3 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.5 +0.7 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 -0.4 +2.0 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -2.7 +3.9 N.D. +0.2
auxotrophy Dropout Complete +0.4 -0.7 +2.0 N.D. -0.0
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