Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9079 and 9080 are separated by 210 nucleotides9080 and 9081 are separated by 1199 nucleotides9081 and 9082 are separated by 125 nucleotides9082 and 9083 are separated by 355 nucleotides mRNA_711: 9079 - K00972 UAP1 UDP-N-acetylglucosamine/UDP-N-acetylgalactosamine diphosphorylase, at 1,700,499 to 1,702,038 9079 mRNA_712: 9080 - KOG4103 Mitochondrial F1F0-ATP synthase, subunit g/ATP20, at 1,702,249 to 1,703,188 9080 mRNA_713: 9081 - Hypothetical Protein, at 1,704,388 to 1,704,930 9081 mRNA_714: 9082 - KOG1201 Hydroxysteroid 17-beta dehydrogenase 11, at 1,705,056 to 1,706,530 9082 mRNA_715: 9083 - KOG2893 Zn finger protein, at 1,706,886 to 1,708,484 9083
Group Condition mRNA_711 mRNA_712 mRNA_713 mRNA_714 mRNA_715
9079 9080 9081 9082 9083
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.9 -0.9 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.9 -0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.8 -0.4 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +1.4 -1.4 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.9 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.5 +0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.2 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.5 +0.5 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.4 -0.2 +0.6
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.5 +0.3 +0.4
growth on oleic acid YNB Glucose N.D. N.D. -0.5 +0.2 +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 -0.0 +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.6 +0.1 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.5 +0.3 +0.6
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 +0.1 +0.1
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.4 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.2 +0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 +0.3 +0.8
auxotrophy YNB Glucose N.D. N.D. +0.3 -0.1 +0.8
growth on ypd YPD N.D. N.D. +0.4 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 +1.0 +0.0
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