Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9083 and 9084 are separated by 1383 nucleotides9084 and 9085 are separated by 447 nucleotides9085 and 9086 are separated by 287 nucleotides9086 and 9087 are separated by 428 nucleotides mRNA_715: 9083 - KOG2893 Zn finger protein, at 1,706,886 to 1,708,484 9083 mRNA_716: 9084 - K01663 HIS7 glutamine amidotransferase / cyclase, at 1,709,868 to 1,711,961 9084 mRNA_717: 9085 - K00058 serA, PHGDH D-3-phosphoglycerate dehydrogenase, at 1,712,409 to 1,714,095 9085 mRNA_718: 9086 - KOG2776 Metallopeptidase, at 1,714,383 to 1,716,364 9086 mRNA_719: 9087 - HMMPfam-Protein of unknown function (DUF3074)-PF11274, at 1,716,793 to 1,718,081 9087
Group Condition mRNA_715 mRNA_716 mRNA_717 mRNA_718 mRNA_719
9083 9084 9085 9086 9087
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. -0.7 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. N.D. -0.5 -0.8
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.5 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. -0.6 +0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. N.D. -1.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. N.D. -0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. N.D. +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. N.D. -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. -0.2 +0.0
growth on oleic acid YNB Oleic Acid +0.4 N.D. N.D. -0.4 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.8 N.D. N.D. -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.4 -0.2
auxotrophy YNB Glucose +0.8 N.D. N.D. -0.3 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 N.D. N.D. +0.2 -0.3
auxotrophy Dropout Complete +0.9 N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 N.D. N.D. +0.3 -0.4
growth on oleic acid YNB Glucose +0.5 N.D. N.D. +0.0 +0.3
growth on ypd YPD +0.4 N.D. N.D. +0.5 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. N.D. +0.8 -0.4
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