Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9086 and 9087 are separated by 428 nucleotides9087 and 9088 are separated by 779 nucleotides9088 and 9089 are separated by 115 nucleotides9089 and 9090 are separated by 552 nucleotides mRNA_718: 9086 - KOG2776 Metallopeptidase, at 1,714,383 to 1,716,364 9086 mRNA_719: 9087 - HMMPfam-Protein of unknown function (DUF3074)-PF11274, at 1,716,793 to 1,718,081 9087 mRNA_720: 9088 - K00993 EPT1 ethanolaminephosphotransferase, at 1,718,861 to 1,720,406 9088 mRNA_721: 9089 - Hypothetical Protein, at 1,720,522 to 1,720,982 9089 mRNA_722: 9090 - HMMPfam-Thiopurine S-methyltransferase (TPMT)-PF05724,ProSiteProfiles-Thiopurine or thiol or thiocyanate S-methyltransferase (TPMT) family profile.-PS51585,SUPERFAMILY--SSF53335, at 1,721,535 to 1,722,519 9090
Group Condition mRNA_718 mRNA_719 mRNA_720 mRNA_721 mRNA_722
9086 9087 9088 9089 9090
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.8 -0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.3 -0.5 N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.4 -0.6 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.4 -0.9 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -1.0 -0.1 -0.3 N.D. +0.3
growth on ricinoleic acid YNB Glucose -0.5 -0.5 -0.5 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.2 -0.6 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine -0.5 -0.1 -0.1 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 -0.4 +0.3 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.2 -0.0 N.D. +0.1
auxotrophy Dropout Complete -0.2 -0.2 -0.2 N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.7 +0.3 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.0 -0.1 N.D. +0.2
auxotrophy YNB Glucose -0.3 +0.0 -0.1 N.D. +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 +0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.1 +0.2 N.D. +0.5
growth on ypd YPD +0.5 -0.0 +0.5 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.4 +0.2 +0.4 N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.1 -0.3 N.D. +0.9
growth on oleic acid YNB Glucose +0.0 +0.3 +0.0 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 +0.2 N.D. +0.8
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