Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9088 and 9089 are separated by 115 nucleotides9089 and 9090 are separated by 552 nucleotides9090 and 9091 are separated by 472 nucleotides9091 and 9092 are separated by 257 nucleotides mRNA_720: 9088 - K00993 EPT1 ethanolaminephosphotransferase, at 1,718,861 to 1,720,406 9088 mRNA_721: 9089 - Hypothetical Protein, at 1,720,522 to 1,720,982 9089 mRNA_722: 9090 - HMMPfam-Thiopurine S-methyltransferase (TPMT)-PF05724,ProSiteProfiles-Thiopurine or thiol or thiocyanate S-methyltransferase (TPMT) family profile.-PS51585,SUPERFAMILY--SSF53335, at 1,721,535 to 1,722,519 9090 mRNA_723: 9091 - K07127 uraH, pucM, hiuH 5-hydroxyisourate hydrolase, at 1,722,992 to 1,724,496 9091 mRNA_724: 9092 - HMMPfam-Ion transport protein-PF00520,SUPERFAMILY--SSF81324, at 1,724,754 to 1,725,732 9092
Group Condition mRNA_720 mRNA_721 mRNA_722 mRNA_723 mRNA_724
9088 9089 9090 9091 9092
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. -0.5 +0.1 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.9 N.D. -0.8 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 N.D. -0.4 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. -0.6 +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.1 -0.5 -0.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.4 -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 -0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.9 -1.0 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. +0.1 -0.4 -0.1
auxotrophy Dropout Complete -0.2 N.D. +0.4 -0.6 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -0.5 +0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.0 -0.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.2 -1.0 +0.1
growth on oleic acid YNB Glucose +0.0 N.D. +0.4 -0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.1 -0.4 +0.0
growth on oleic acid YNB Oleic Acid +0.4 N.D. +0.4 -0.5 -0.3
growth on ypd YPD +0.5 N.D. -0.4 -0.1 +0.2
auxotrophy YNB Glucose -0.1 N.D. +0.7 -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.5 -0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. +0.3 +0.8 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.8 -0.1 -0.0
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