Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9098 and 9099 are separated by 314 nucleotides9099 and 9100 are separated by 382 nucleotides9100 and 9101 are separated by 42 nucleotides9101 and 9102 are separated by 395 nucleotides mRNA_730: 9098 - KOG0061 Transporter, ABC superfamily (Breast cancer resistance protein), at 1,738,976 to 1,743,135 9098 mRNA_731: 9099 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 1,743,450 to 1,746,549 9099 mRNA_732: 9100 - HMMPfam-Dioxygenase-PF00775,HMMPfam-Catechol dioxygenase N terminus-PF04444,SUPERFAMILY--SSF49482, at 1,746,932 to 1,748,724 9100 mRNA_733: 9101 - K13954 yiaY alcohol dehydrogenase, at 1,748,767 to 1,750,473 9101 mRNA_734: 9102 - K08149 SLC2A12, GLUT12 MFS transporter, SP family, solute carrier family 2 (facilitated glucose transporter), member 12, at 1,750,869 to 1,753,209 9102
Group Condition mRNA_730 mRNA_731 mRNA_732 mRNA_733 mRNA_734
9098 9099 9100 9101 9102
facs on lipid accumulation Sorted High BODIPY Gate -1.0 -0.2 -0.2 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -0.2 +0.2 -0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.0 -0.2 +0.1 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.1 +0.3 -0.1 -0.1
auxotrophy Dropout Complete +0.4 -0.2 -0.3 -0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.2 +0.3 -0.6 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.0 -0.1 -0.2 -0.4
growth on ricinoleic acid YNB Glucose -0.0 -0.2 -0.0 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 +0.2 +0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.1 -0.1 +0.4 +0.1
growth on ypd YPD -0.0 +0.1 +0.4 -0.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.1 -0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 +0.2 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 -0.1 +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.0 -0.2 +0.1 +0.1
auxotrophy YNB Glucose +0.3 +0.3 -0.0 -0.1 -0.4
growth on oleic acid YNB Glucose +0.2 +0.2 +0.2 +0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 -0.3 +0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.3 +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 +0.1 +0.2 +0.0
growth on oleic acid YNB Oleic Acid +0.3 +0.3 +0.0 +0.1 +0.1
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