Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9106 and 9107 are separated by 1800 nucleotides9107 and 9108 are separated by 230 nucleotides9108 and 9109 are separated by 153 nucleotides9109 and 9110 are separated by 183 nucleotides mRNA_738: 9106 - K06606 iolI 2-keto-myo-inositol isomerase, at 1,757,999 to 1,760,705 9106 mRNA_739: 9107 - K07359 CAMKK calcium/calmodulin-dependent protein kinase kinase, at 1,762,506 to 1,766,867 9107 mRNA_740: 9108 - K13103 TFIP11 tuftelin-interacting protein 11, at 1,767,098 to 1,770,950 9108 mRNA_741: 9109 - K12858 DDX23, PRP28 ATP-dependent RNA helicase DDX23/PRP28, at 1,771,104 to 1,774,231 9109 mRNA_742: 9110 - HMMPfam-SPT2 chromatin protein-PF08243,SMART-SPT2 chromatin protein-SM00784, at 1,774,415 to 1,775,869 9110
Group Condition mRNA_738 mRNA_739 mRNA_740 mRNA_741 mRNA_742
9106 9107 9108 9109 9110
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -1.5 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.9 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -1.0 N.D. N.D. +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.4 N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.3 N.D. N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.4 N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.6 N.D. N.D. -0.2
growth on ypd YPD +0.0 -0.4 N.D. N.D. +0.0
auxotrophy Dropout Complete +0.3 -0.5 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.1 N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.4 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 N.D. N.D. -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.1 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.0 N.D. N.D. -0.1
auxotrophy YNB Glucose +0.1 +0.0 N.D. N.D. -0.0
growth on oleic acid YNB Glucose -0.1 -0.0 N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.2 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.2 N.D. N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.2 N.D. N.D. +0.2
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