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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9112 and 9113 are separated by 197 nucleotides
9113 and 9114 are separated by 332 nucleotides
9114 and 9115 are separated by 158 nucleotides
9115 and 9116 are separated by 948 nucleotides
mRNA_744: 9112 - KOG2533 Permease of the major facilitator superfamily, at 1,777,917 to 1,780,253
9112
mRNA_745: 9113 - K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome), at 1,780,451 to 1,782,779
9113
mRNA_746: 9114 - KOG0143 Iron/ascorbate family oxidoreductases, at 1,783,112 to 1,784,758
9114
mRNA_747: 9115 - K02737 PSMB5 20S proteasome subunit beta 5, at 1,784,917 to 1,786,577
9115
mRNA_748: 9116 - Hypothetical Protein, at 1,787,526 to 1,789,386
9116
Group
Condition
mRNA
_744
mRNA
_745
mRNA
_746
mRNA
_747
mRNA
_748
9112
9113
9114
9115
9116
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
-0.3
-0.0
N.D.
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
-0.1
-0.5
N.D.
-0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.3
+0.2
-0.5
N.D.
-0.6
ypd buoyancy
40 hrs High Buoyancy
-0.6
+0.3
+0.2
N.D.
-0.1
auxotrophy
Dropout Complete
-0.8
-0.2
+0.2
N.D.
+0.6
facs on lipid accumulation
Sorted No BODIPY Gate
+0.4
-0.1
-0.1
N.D.
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.0
-0.0
+0.2
N.D.
-0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.0
+0.1
+0.2
N.D.
-0.1
growth on oleic acid
YNB Glucose
-0.7
+0.2
+0.3
N.D.
+0.4
auxotrophy
YNB Glucose 75mM Methionine
-0.2
+0.1
+0.0
N.D.
+0.6
auxotrophy
YNB Glucose 75mM Arginine
-0.6
+0.1
+0.3
N.D.
+0.8
growth on ypd
YPD
-0.3
+0.2
+0.6
N.D.
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.3
+0.4
-0.1
N.D.
+0.6
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
+0.2
+0.4
N.D.
+0.4
growth on oleic acid
YNB Oleic Acid
+0.0
+0.0
+0.3
N.D.
+0.4
auxotrophy
YNB Glucose
-0.4
+0.4
+0.3
N.D.
+0.5
growth on ricinoleic acid
YNB Glucose
-0.0
+0.3
-0.1
N.D.
+0.7
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.0
+0.4
-0.3
N.D.
+0.8
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
+0.3
+0.4
N.D.
+0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.3
+0.4
+0.3
N.D.
+0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.0
+0.4
+0.6
N.D.
+0.4
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