Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9169 and 9170 are separated by 513 nucleotides9170 and 9171 are separated by 390 nucleotides9171 and 9172 are separated by 1510 nucleotides9172 and 9173 are separated by 434 nucleotides mRNA_801: 9169 - SUPERFAMILY--SSF52047, at 1,905,465 to 1,906,798 9169 mRNA_802: 9170 - SUPERFAMILY--SSF52047, at 1,907,312 to 1,908,570 9170 mRNA_803: 9171 - HMMPfam-Velvet factor-PF11754, at 1,908,961 to 1,910,410 9171 mRNA_804: 9172 - K17794 TIM23 mitochondrial import inner membrane translocase subunit TIM23, at 1,911,921 to 1,912,961 9172 mRNA_805: 9173 - K09191 GTF3A general transcription factor IIIA, at 1,913,396 to 1,915,422 9173
Group Condition mRNA_801 mRNA_802 mRNA_803 mRNA_804 mRNA_805
9169 9170 9171 9172 9173
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.5 -0.9 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -1.5 +0.0 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.6 -1.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.0 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.4 +0.0 N.D. N.D.
auxotrophy YNB Glucose -0.1 +0.3 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.2 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.6 -0.9 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.4 +0.0 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.4 +0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.0 +1.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.3 +0.3 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.5 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.6 -0.4 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 +0.3 N.D. N.D.
growth on oleic acid YNB Glucose +0.4 +0.3 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.7 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.4 +1.4 N.D. N.D.
growth on ypd YPD +0.4 +1.6 -0.3 N.D. N.D.
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