Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9171 and 9172 are separated by 1510 nucleotides9172 and 9173 are separated by 434 nucleotides9173 and 9174 are separated by 273 nucleotides9174 and 9175 are separated by 608 nucleotides mRNA_803: 9171 - HMMPfam-Velvet factor-PF11754, at 1,908,961 to 1,910,410 9171 mRNA_804: 9172 - K17794 TIM23 mitochondrial import inner membrane translocase subunit TIM23, at 1,911,921 to 1,912,961 9172 mRNA_805: 9173 - K09191 GTF3A general transcription factor IIIA, at 1,913,396 to 1,915,422 9173 mRNA_806: 9174 - KOG1515 Arylacetamide deacetylase, at 1,915,696 to 1,917,845 9174 mRNA_807: 9175 - Hypothetical Protein, at 1,918,454 to 1,920,655 9175
Group Condition mRNA_803 mRNA_804 mRNA_805 mRNA_806 mRNA_807
9171 9172 9173 9174 9175
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 N.D. N.D. -0.0 -0.4
auxotrophy Dropout Complete -1.1 N.D. N.D. +0.2 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 N.D. N.D. -0.2 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.6 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. N.D. +0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. -0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.3 -0.3
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.3 -0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. +0.1 -0.0
growth on ypd YPD -0.3 N.D. N.D. +0.3 +0.2
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. N.D. +0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. N.D. -0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. +0.5 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 N.D. N.D. +0.3 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.4 N.D. N.D. +0.1 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.8 N.D. N.D. +0.2 +0.0
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