Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9179 and 9180 are separated by 472 nucleotides9180 and 9181 are separated by 261 nucleotides9181 and 9182 are separated by 522 nucleotides9182 and 9183 are separated by 127 nucleotides mRNA_811: 9179 - Interpro Glycosyl-transferase for dystroglycan, at 1,929,114 to 1,931,965 9179 mRNA_812: 9180 - K00474 TMLHE trimethyllysine dioxygenase, at 1,932,438 to 1,934,306 9180 mRNA_813: 9181 - KOG1515 Arylacetamide deacetylase, at 1,934,568 to 1,937,031 9181 mRNA_814: 9182 - K15075 MET18, MMS19 DNA repair/transcription protein MET18/MMS19, at 1,937,554 to 1,941,739 9182 mRNA_815: 9183 - K04555 UBE2G2, UBC7 ubiquitin-conjugating enzyme E2 G2, at 1,941,867 to 1,942,865 9183
Group Condition mRNA_811 mRNA_812 mRNA_813 mRNA_814 mRNA_815
9179 9180 9181 9182 9183
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. -0.3 N.D. -2.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. +0.2 N.D. -2.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.2 N.D. -1.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 N.D. -0.0 N.D. -1.0
growth on oleic acid YNB Oleic Acid +1.2 N.D. +0.2 N.D. -3.4
ypd buoyancy 40 hrs No Buoyancy Separation -1.8 N.D. +0.3 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +2.0 N.D. -0.2 N.D. -3.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. -0.2 N.D. -2.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.0 N.D. -1.2
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. +0.2 N.D. -2.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.1 N.D. -1.2
growth on ricinoleic acid YNB Ricinoleic Acid +2.2 N.D. +0.3 N.D. -3.6
growth on oleic acid YNB Glucose +0.5 N.D. +0.2 N.D. -1.7
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.4 N.D. -1.6
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. -0.0 N.D. -1.0
ypd buoyancy 40 hrs Low Buoyancy +0.7 N.D. -0.1 N.D. -1.4
growth on ricinoleic acid YNB Glucose +1.1 N.D. -0.1 N.D. -1.7
auxotrophy YNB Glucose -0.1 N.D. +0.2 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 N.D. -0.1
auxotrophy Dropout Complete +0.0 N.D. +0.4 N.D. -0.1
growth on ypd YPD -0.1 N.D. +0.0 N.D. +1.0
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