Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9180 and 9181 are separated by 261 nucleotides9181 and 9182 are separated by 522 nucleotides9182 and 9183 are separated by 127 nucleotides9183 and 9184 are separated by 735 nucleotides mRNA_812: 9180 - K00474 TMLHE trimethyllysine dioxygenase, at 1,932,438 to 1,934,306 9180 mRNA_813: 9181 - KOG1515 Arylacetamide deacetylase, at 1,934,568 to 1,937,031 9181 mRNA_814: 9182 - K15075 MET18, MMS19 DNA repair/transcription protein MET18/MMS19, at 1,937,554 to 1,941,739 9182 mRNA_815: 9183 - K04555 UBE2G2, UBC7 ubiquitin-conjugating enzyme E2 G2, at 1,941,867 to 1,942,865 9183 mRNA_816: 9184 - KOG0783 Uncharacterized conserved protein, contains ankyrin and BTB/POZ domains, at 1,943,601 to 1,948,343 9184
Group Condition mRNA_812 mRNA_813 mRNA_814 mRNA_815 mRNA_816
9180 9181 9182 9183 9184
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. -3.6 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. -3.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. -3.6 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. -2.5 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 N.D. -2.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. -2.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. -1.8 +0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -1.7 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. -2.0 +0.2
growth on oleic acid YNB Glucose N.D. +0.2 N.D. -1.7 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -1.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 N.D. -1.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. -1.2 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 N.D. -1.6 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 N.D. -1.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -1.2 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. -0.1 -0.1
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.5 +0.3
auxotrophy Dropout Complete N.D. +0.4 N.D. -0.1 -0.1
growth on ypd YPD N.D. +0.0 N.D. +1.0 -0.2
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