Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9181 and 9182 are separated by 522 nucleotides9182 and 9183 are separated by 127 nucleotides9183 and 9184 are separated by 735 nucleotides9184 and 9185 are separated by 364 nucleotides mRNA_813: 9181 - KOG1515 Arylacetamide deacetylase, at 1,934,568 to 1,937,031 9181 mRNA_814: 9182 - K15075 MET18, MMS19 DNA repair/transcription protein MET18/MMS19, at 1,937,554 to 1,941,739 9182 mRNA_815: 9183 - K04555 UBE2G2, UBC7 ubiquitin-conjugating enzyme E2 G2, at 1,941,867 to 1,942,865 9183 mRNA_816: 9184 - KOG0783 Uncharacterized conserved protein, contains ankyrin and BTB/POZ domains, at 1,943,601 to 1,948,343 9184 mRNA_817: 9185 - K02208 CDK8_11 cyclin-dependent kinase 8/11, at 1,948,708 to 1,950,488 9185
Group Condition mRNA_813 mRNA_814 mRNA_815 mRNA_816 mRNA_817
9181 9182 9183 9184 9185
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -2.5 -0.0 -2.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -3.6 +0.3 -1.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -3.6 +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -2.2 -0.2 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -2.1 +0.1 -0.5
growth on oleic acid YNB Oleic Acid +0.2 N.D. -3.4 +0.1 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -1.8 +0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -1.0 -0.1 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -1.4 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -1.2 +0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. -1.7 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -1.0 +0.1 -0.1
growth on oleic acid YNB Glucose +0.2 N.D. -1.7 +0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -0.4 +0.0 -0.7
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. -2.0 +0.2 +0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -1.2 +0.4 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -1.6 +0.1 +0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.1 -0.3
auxotrophy YNB Glucose +0.2 N.D. -0.5 +0.3 +0.0
growth on ypd YPD +0.0 N.D. +1.0 -0.2 -0.5
auxotrophy Dropout Complete +0.4 N.D. -0.1 -0.1 +0.4
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