Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9182 and 9183 are separated by 127 nucleotides9183 and 9184 are separated by 735 nucleotides9184 and 9185 are separated by 364 nucleotides9185 and 9186 are separated by 158 nucleotides mRNA_814: 9182 - K15075 MET18, MMS19 DNA repair/transcription protein MET18/MMS19, at 1,937,554 to 1,941,739 9182 mRNA_815: 9183 - K04555 UBE2G2, UBC7 ubiquitin-conjugating enzyme E2 G2, at 1,941,867 to 1,942,865 9183 mRNA_816: 9184 - KOG0783 Uncharacterized conserved protein, contains ankyrin and BTB/POZ domains, at 1,943,601 to 1,948,343 9184 mRNA_817: 9185 - K02208 CDK8_11 cyclin-dependent kinase 8/11, at 1,948,708 to 1,950,488 9185 mRNA_818: 9186 - HMMPfam-PQ loop repeat-PF04193, at 1,950,647 to 1,952,582 9186
Group Condition mRNA_814 mRNA_815 mRNA_816 mRNA_817 mRNA_818
9182 9183 9184 9185 9186
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -3.6 +0.3 -1.2 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. -2.5 -0.0 -2.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -2.2 -0.2 -0.7 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -3.6 +0.1 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 +0.0 -0.7 -1.6
growth on oleic acid YNB Oleic Acid N.D. -3.4 +0.1 +0.8 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -1.4 +0.1 -0.2 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -2.1 +0.1 -0.5 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 -0.1 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.8 +0.0 -0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -1.2 +0.1 -0.2 +0.1
growth on oleic acid YNB Glucose N.D. -1.7 +0.1 +0.5 -0.1
growth on ricinoleic acid YNB Glucose N.D. -1.7 +0.2 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -1.2 +0.4 +0.2 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -2.0 +0.2 +0.8 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.1 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.0 +0.1 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -1.6 +0.1 +0.6 +0.4
auxotrophy YNB Glucose N.D. -0.5 +0.3 +0.0 +0.3
auxotrophy Dropout Complete N.D. -0.1 -0.1 +0.4 +0.2
growth on ypd YPD N.D. +1.0 -0.2 -0.5 +0.2
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