Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9188 and 9189 are separated by 725 nucleotides9189 and 9190 are separated by 549 nucleotides9190 and 9191 are separated by 133 nucleotides9191 and 9192 are separated by 373 nucleotides mRNA_820: 9188 - K20495 CYP704B1 long-chain fatty acid omega-monooxygenase, at 1,958,610 to 1,960,839 9188 mRNA_821: 9189 - K14301 NUP107, NUP84 nuclear pore complex protein Nup107, at 1,961,565 to 1,964,829 9189 mRNA_822: 9190 - HMMPfam-Sec7 domain-PF01369,HMMPfam-Pleckstrin homology domain-PF15410,ProSiteProfiles-PH domain profile.-PS50003,ProSiteProfiles-SEC7 domain profile.-PS50190,SMART-Sec7 domain-SM00222,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF48425,SUPERFAMILY--SSF50729, at 1,965,379 to 1,971,450 9190 mRNA_823: 9191 - HMMPfam-Fungal domain of unknown function (DUF1712)-PF08217, at 1,971,584 to 1,974,293 9191 mRNA_824: 9192 - Hypothetical Protein, at 1,974,667 to 1,975,574 9192
Group Condition mRNA_820 mRNA_821 mRNA_822 mRNA_823 mRNA_824
9188 9189 9190 9191 9192
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.2 +0.0 -0.9
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.7 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.1 -0.2 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.9 +0.3 -0.1
auxotrophy Dropout Complete +0.2 N.D. -0.4 +0.2 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. -0.4 -0.0 -0.6
growth on ypd YPD -0.4 N.D. -0.2 -0.4 +0.7
auxotrophy YNB Glucose +0.6 N.D. -0.2 +0.3 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.3 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.1 +0.2 -0.2
growth on oleic acid YNB Glucose +0.6 N.D. -0.3 +0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.1 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 +0.4 -0.2
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.5 +0.0 +0.6
growth on oleic acid YNB Oleic Acid +0.8 N.D. +0.1 -0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 N.D. -0.2 +0.0 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 N.D. -0.0 +0.0 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 +0.7 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. +0.1 +0.7 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.1 +1.0 +0.6
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