Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9189 and 9190 are separated by 549 nucleotides9190 and 9191 are separated by 133 nucleotides9191 and 9192 are separated by 373 nucleotides9192 and 9193 are separated by 469 nucleotides mRNA_821: 9189 - K14301 NUP107, NUP84 nuclear pore complex protein Nup107, at 1,961,565 to 1,964,829 9189 mRNA_822: 9190 - HMMPfam-Sec7 domain-PF01369,HMMPfam-Pleckstrin homology domain-PF15410,ProSiteProfiles-PH domain profile.-PS50003,ProSiteProfiles-SEC7 domain profile.-PS50190,SMART-Sec7 domain-SM00222,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF48425,SUPERFAMILY--SSF50729, at 1,965,379 to 1,971,450 9190 mRNA_823: 9191 - HMMPfam-Fungal domain of unknown function (DUF1712)-PF08217, at 1,971,584 to 1,974,293 9191 mRNA_824: 9192 - Hypothetical Protein, at 1,974,667 to 1,975,574 9192 mRNA_825: 9193 - HMMPfam-Modifier of rudimentary (Mod(r)) protein-PF07200,ProSiteProfiles-VPS37 C-terminal domain profile.-PS51314,SUPERFAMILY--SSF140111, at 1,976,044 to 1,976,786 9193
Group Condition mRNA_821 mRNA_822 mRNA_823 mRNA_824 mRNA_825
9189 9190 9191 9192 9193
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 -0.1 +0.1 -4.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.9 +0.3 -0.1 -4.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.0 -0.6 -3.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 +0.3 +0.2 -4.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.0 +0.4 -4.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 +0.0 -0.9 -2.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.0 +0.4 -3.1
auxotrophy YNB Glucose N.D. -0.2 +0.3 -0.6 -2.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.4 -0.2 -2.7
growth on ricinoleic acid YNB Glucose N.D. -0.5 +0.0 +0.6 -2.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 +0.7 +0.2 -2.5
auxotrophy Dropout Complete N.D. -0.4 +0.2 -0.5 -0.7
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 +0.2 -0.2 -1.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.2 -0.5 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.7 -0.1 -1.7
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.2 -0.2 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.2 +0.2 -0.3
growth on oleic acid YNB Glucose N.D. -0.3 +0.0 -0.1 +0.3
growth on ypd YPD N.D. -0.2 -0.4 +0.7 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.0 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +1.0 +0.6 -0.8
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