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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9208 and 9209 are separated by 758 nucleotides
9209 and 9210 are separated by 195 nucleotides
9210 and 9211 are separated by 107 nucleotides
9211 and 9212 are separated by 257 nucleotides
mRNA_840: 9208 - BLAST protein of unknown function DUF1746, fungi [Rhodo..., at 2,012,054 to 2,014,320
9208
mRNA_841: 9209 - HMMPfam-Kinetochore protein CHL4 like-PF05238, at 2,015,079 to 2,016,824
9209
mRNA_842: 9210 - K02148 ATPeV1C, ATP6C V-type H+-transporting ATPase subunit C, at 2,017,020 to 2,018,936
9210
mRNA_843: 9211 - KOG2262 Sexual differentiation process protein ISP4, at 2,019,044 to 2,022,236
9211
mRNA_844: 9212 - KOG1111 N-acetylglucosaminyltransferase complex, subunit PIG-A/SPT14, required for phosphatidylinositol biosynthesis/Sulfolipid synthase, at 2,022,494 to 2,024,852
9212
Group
Condition
mRNA
_840
mRNA
_841
mRNA
_842
mRNA
_843
mRNA
_844
9208
9209
9210
9211
9212
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.7
-0.0
N.D.
-0.1
-0.2
auxotrophy
Dropout Complete
-0.4
+0.4
N.D.
-0.1
-0.6
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.7
-0.1
N.D.
+0.4
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-1.2
+0.3
N.D.
-0.0
+0.4
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.9
-0.0
N.D.
+0.4
-0.0
auxotrophy
YNB Glucose 75mM Methionine
-0.6
+0.4
N.D.
-0.1
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.5
+0.2
N.D.
+0.0
-0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
-0.1
N.D.
+0.1
-0.5
auxotrophy
YNB Glucose 75mM Arginine
-0.5
+0.1
N.D.
+0.0
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
+0.0
N.D.
+0.2
-0.6
ypd buoyancy
40 hrs Low Buoyancy
+0.3
-0.3
N.D.
+0.0
-0.0
growth on oleic acid
YNB Glucose
-0.7
+0.2
N.D.
+0.5
+0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.1
N.D.
+0.1
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
+0.5
N.D.
-0.3
+0.3
growth on ypd
YPD
+0.7
-0.2
N.D.
-0.1
-0.1
growth on ricinoleic acid
YNB Glucose
-0.3
+0.1
N.D.
+0.5
+0.1
auxotrophy
YNB Glucose
-0.2
+0.2
N.D.
+0.3
+0.1
growth on oleic acid
YNB Oleic Acid
-0.1
+0.2
N.D.
+0.0
+0.3
ypd buoyancy
40 hrs High Buoyancy
+0.3
+0.0
N.D.
+0.1
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
+0.5
-0.2
N.D.
-0.1
+0.4
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
+0.1
N.D.
+0.2
+0.7
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