Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9211 and 9212 are separated by 257 nucleotides9212 and 9213 are separated by 568 nucleotides9213 and 9214 are separated by 774 nucleotides9214 and 9215 are separated by 265 nucleotides mRNA_843: 9211 - KOG2262 Sexual differentiation process protein ISP4, at 2,019,044 to 2,022,236 9211 mRNA_844: 9212 - KOG1111 N-acetylglucosaminyltransferase complex, subunit PIG-A/SPT14, required for phosphatidylinositol biosynthesis/Sulfolipid synthase, at 2,022,494 to 2,024,852 9212 mRNA_845: 9213 - KOG3346 Phosphatidylethanolamine binding protein, at 2,025,421 to 2,026,759 9213 mRNA_846: 9214 - KOG2710 Rho GTPase-activating protein, at 2,027,534 to 2,029,466 9214 mRNA_847: 9215 - HMMPfam-Amino acid permease-PF13520, at 2,029,732 to 2,032,618 9215
Group Condition mRNA_843 mRNA_844 mRNA_845 mRNA_846 mRNA_847
9211 9212 9213 9214 9215
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.5 -0.2 -0.9 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.3 -0.2 -0.5 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.1 -0.5 -0.1
auxotrophy Dropout Complete -0.1 -0.6 +0.3 -0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.4 +0.0 -0.8 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.3 -0.3 +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 +0.0 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.6 -0.2 +0.8 -0.4
growth on ypd YPD -0.1 -0.1 -0.2 +0.4 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.3 -0.1 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.0 -0.2 +0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 +0.0 +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.1 +0.2 -0.3 +0.2
growth on oleic acid YNB Oleic Acid +0.0 +0.3 +0.0 -0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 -0.1 -0.0 +0.3
auxotrophy YNB Glucose +0.3 +0.1 +0.2 -0.1 +0.0
growth on oleic acid YNB Glucose +0.5 +0.0 -0.0 -0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.7 +0.0 -0.7 +0.3
growth on ricinoleic acid YNB Glucose +0.5 +0.1 +0.1 +0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.0 +0.2 +0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.4 +0.1 +0.2 +0.2
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