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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9211 and 9212 are separated by 257 nucleotides
9212 and 9213 are separated by 568 nucleotides
9213 and 9214 are separated by 774 nucleotides
9214 and 9215 are separated by 265 nucleotides
mRNA_843: 9211 - KOG2262 Sexual differentiation process protein ISP4, at 2,019,044 to 2,022,236
9211
mRNA_844: 9212 - KOG1111 N-acetylglucosaminyltransferase complex, subunit PIG-A/SPT14, required for phosphatidylinositol biosynthesis/Sulfolipid synthase, at 2,022,494 to 2,024,852
9212
mRNA_845: 9213 - KOG3346 Phosphatidylethanolamine binding protein, at 2,025,421 to 2,026,759
9213
mRNA_846: 9214 - KOG2710 Rho GTPase-activating protein, at 2,027,534 to 2,029,466
9214
mRNA_847: 9215 - HMMPfam-Amino acid permease-PF13520, at 2,029,732 to 2,032,618
9215
Group
Condition
mRNA
_843
mRNA
_844
mRNA
_845
mRNA
_846
mRNA
_847
9211
9212
9213
9214
9215
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
-0.5
-0.2
-0.9
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
-0.3
-0.2
-0.5
-0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
-0.2
-0.1
-0.5
-0.1
auxotrophy
Dropout Complete
-0.1
-0.6
+0.3
-0.4
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.1
+0.4
+0.0
-0.8
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
+0.3
-0.3
+0.1
-0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.1
-0.2
+0.0
-0.2
+0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.2
-0.6
-0.2
+0.8
-0.4
growth on ypd
YPD
-0.1
-0.1
-0.2
+0.4
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.3
-0.1
+0.2
-0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.0
-0.0
-0.2
+0.2
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.1
-0.1
+0.0
+0.0
+0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.0
+0.1
+0.2
-0.3
+0.2
growth on oleic acid
YNB Oleic Acid
+0.0
+0.3
+0.0
-0.0
-0.0
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.0
-0.1
-0.0
+0.3
auxotrophy
YNB Glucose
+0.3
+0.1
+0.2
-0.1
+0.0
growth on oleic acid
YNB Glucose
+0.5
+0.0
-0.0
-0.0
+0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
+0.7
+0.0
-0.7
+0.3
growth on ricinoleic acid
YNB Glucose
+0.5
+0.1
+0.1
+0.0
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.4
-0.0
+0.2
+0.0
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.0
+0.4
+0.1
+0.2
+0.2
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